Group Leader - Prof. Sheena E. Radford, OBE FMedSci FRS
Research Interests: Protein Folding and Misfolding.
BSc in Biochemistry, University of Birmingham; PhD Biochemistry, University of Cambridge; Royal Society University Research Fellow (Protein Folding) in the Oxford Centre for Molecular Sciences; University Lecturer in the School of Biochemistry & Molecular Biology, University of Leeds; Reader in Structural Molecular Biology, University of Leeds; Professor of Structural Molecular Biology, University of Leeds.
Astbury Centre of Structural Molecular Biology, Garstang Building, University of Leeds, Leeds, LS2 9JT
Tel: 0113 343 3170; FAX: 0113 343 3167;
|Our support team|
Helen is Prof Radford's PA. She organises the group and keeps everything coordinated. She can be contacted on firstname.lastname@example.org 0113 343 7036
Nasir joined the group in 2010 as our lab manager. He keeps the lab running smoothly and hence is a key member of our research team. He has excellent skills in maintaining and developing our excellent equipment base and also works as the facility manager for the CD facility.
|The Amyloid Team|
Ms Madeline Brown|
Maddie joined our group in October 2017 funded on a MRC DTP PhD studentship. Cell biology of amyloidosis, specifically how different polymorphs affect cells, is the topic of her PhD. She is co-supervised by Dr Eric Hewitt.
Ms Emily Byrd|
Emily is doing her PhD in biological mass spectrometry of protein aggregation, focussing on alpha-synuclein. Using ion-mobility, hydrogen exchange, lignad binding, mutagenesis, and rapid cross linking, she is using MS methods to elucidate how and why alpha synuclein aggregates in Parkinson's disease. She is co-supervised by Prof Frank Sobott and funded by our White Rose BBSRC DTP. She joined in 2019.
Mr Chalmers Chau|
Chalmers is a self-funded PhD student working on a project to use the nano-pippette to introduce amyloid intermediates into cells so as to study their biological effects. His project is co-supervised with Dr Eric Hewitt and Dr Paolo Actis (School of Electronic and Electrical Engineering). He started in 2017 having completed a BSc at Imperial College, London.
Mr Michael Davies|
Michael is a PhD student funded by the BBSRC White Rose DTP who started in the lab in October 2016. Co-supervised by Dr Eric Hewitt, his project focusses on the cell biology of amyloidosis, particularly how cells respond to the presnce of oligomeric and fibrillar aggregates. He joined us having completed his BSc in Veterinary Science at the Royal Veterinary College.
Ms Katy Dewison|
Funded by the MRC DiMeN Doctoral Training Programme, Katy joined us in November 2020 to investigate protein mis-folding and its toxic consequences by combining biochemistry with experiment in C. elegans. She obtained her Masters at the University of Edinburgh and has a BSc in Molecular Biology. Jointly supervised with Dr Patricija van Oosten Hawle, Katy is working on α-synuclein, amylin and β2-microglobulin amyloidoses.
Dr Rodrigo Gallardo|
Rodrigo is a structrual biologist and biophysicist who joined the group in 2018, completing the amyloid team funded by The Wellcome Trust. He brings significant expertise in amyloid structure and formation, having worked previously in the groups of Joost Schymkowitz and Fred Rousseau at the VIB Switch Laboratory, K.U. Leuven. He is working on using cryo-EM and cryo-ET to analyse amyloid structures formed in vitro and in vivo, making full use of our two Titan Krios EMs in the Astbury Biostructure Laboratory.
Ms Sarah Good|
Sarah started as a PhD student in October 2016 having graduated with a degree in Biological Sciences from the University of Leeds. She is funded by the BBSRC White Rose DTP. Co-supervised by Dr Patricija vanOosten-Hawle she will bring expertise in using C. elegans as a model for amyloid disease and its inhibition using small molecules and other strategies.
Dr Nicolas Guthertz|
Nicolas joined the group in August 2017 having completed his PhD at ICR in London. His skills in protein crystallography and protein-ligand interactions are now being applied to biochemical and kinetic analysis of the early stages in the aggregation of β2-microglobulin and amylin. He is funded by the Wellcome Trust.
Dr Roberto Maya Martinez|
Roberto joined the group in 2018 bringing skills in NMR to the group. He is funded by The Wellcome Trust and is investigating the mechanism of aggregation of proteins into amyloid and how this can be arrested using small molecules or protein affimers. He held previous positions at Grenoble in NMR having undertaken his PhD in Mexico.
Ms Keri Moore|
Welcome to the amyloid team, Keri! For your MBiol research project 2020-2021. Keri is working with Sabine and Rodrigo on determining amyloid structures using cryo-EM.
Mr Tim Peace|
Tim is a self-funded American PhD student who is co-supervised by Drs Paolo Actis and Eric Hewitt, using and developing nano-pippettes for the analysis of the toxic properties of amyloid aggregates in cells and neurones. He joined us in November 2020. Tim completed his BA in biology at the University of Maine at Presque Isle (UMPI) before a MSc in neuroscience at the University of Sheffield.
Ms Sabine Ulamec|
Sabine is funded by the Wellcome Trust 4-year PhD programme and has chosen to study the mechanism of amyloid formation for her PhD project. She joined the group for her PhD project in summer 2018 and is co-supervised by David Brockwell. Using NMR, kinetic analyses and cryo-EM her project aims to map how α-synuclein forms amyloid in molecular detail.
Dr Martin Walko|
Martin joined the group in 2017 funded by EPSRC. His project is collaborative with Prof Andy Wilson (Chemistry), Prof Alison Ashcroft (mass spec) and Dr Eric Hewitt (cell biology). Martin's synthetic chemistry skills are being used to synthesise Aβ40/42 containing amino acids modified by cross linkers. His project aims to determine the structure and toxicity of Aβ aggregation intermediates.
Dr Martin Wilkinson|
Martin joined the amyloid team in October 2020 having worked previously in structural biology at Imperial College London. He is funded by the MR with Prof Neil Ranson and his project is focussing on using cryo-EM to determine the structure of amyloid fibrils.
Dr Yong Xu|
Yong brings skills in medicinal chemistry to the group and is part of the amyloid team funded by The Wellcome Trust. He completed his PhD at Imperial College London. He is screening and designing small molecules able to arrest amyloid formation at different stages of assembly.
|The OMP Team|
Mr Sam Haysom|
Sam joined the lab in Oct 2018 having completed his BSc in Biochemistry at the University of York. Funded by the White Rose BBSRC DTP, he is co-supervised by Prof Neil Ranson and is working on the structure and functional dynamics of the β-barrel assembly machinery (BAM) in the biogenesis of the bacterial cell envelop using cryo-EM.
Dr Robert Schiffrin|
Bob graduated in Biochemistry at the University of Leeds in July 2012 and joined the group as a PhD student to study the mechanisms of outer membrane protein folding and the involvement of molecular chaperones in this process. Jointly supervised by Dr David Brockwell, he is now a postdoc funded by BBSRC and is studying the molecular mechanism of BAM using a suite of biophysical approaches.
Dr Matt Watson|
Funded by BBSRC, Matt is our expert in single molecule FRET. Working collaboratively with Profs Roman Tuma (University of South Bohemia, Czech Republic) and Ian Collinson (University of Bristol) Matt is investigating the mechanism of action of SecYEG and how BAM catalyses OMP folding.
Dr Paul White|
Paul graduated from the University of Leeds in Biochemistry and then undertook a DPhil in structural biology at the University of Oxford funded by the Wellcome Trust. He joined the group in November 2017. Working alongside Matt Iadanza and the OMP team, Paul's project is to elucidate structural insights into how BAM folds OMPs into the outer membrane. He is funded on a MRC programme grant jointly with Prof Neil Ranson.
Mr James Whitehouse|
James joined the group in Oct 2018 having graduated in Biochemistry from the University of Birmingham. Funded by a large EOS grant with colleague in Belgium, James has joined our OMP-team, and his project will investigate the mechanism of folding and chaperoning of OMPs into the bacterial outer membrane catalysed by BAM
|The Biologics Team|
Mr Miles Deans|
Miles studied at the University of Oxford, gaining his degree in 2020, before joining us to work on protein aggregation mechanisms with Prof David Brockwell. He brings us skills in bioinformatics and computational biology and will be combining experimental and computational methods to aid the prediction of protein aggregation especially of relevance to biopharma. He is funded by a BBSRC iCASE studentship with AstraZeneca.
Ms Maia Harvey|
We have developed a split β-lactamase system to evolve proteins with enhanced stability or to reduce their aggregation propensity. Funded on the BBSRC White Rose DTP from October 2017, and co-supervised by Drs David Brockwell and Darren Tomlinson, Maia's project will develop the system further to screen for modulators of protein-protein interactions in vivo.
Ms Sammy Lawrence|
Sammy is working with Sheena Radford, David Brockwell and Nic Kapur investigating how flow causes proteins to aggregate with a focus on antibodies and antibody-like molecules. She is funded by the BBSRC on a CASE award with Medimmune plc and her project will focus on the use of advanced mass spectrometric methods to interrogate how proteins unfold under flow.
Ms Romany McLure|
Romany joined the group in Oct 2018, funded on a BBSRC CASE award in partnership with UCB. She is co-supervised by David Brockwell. He research focus is to use evolutionary methods to explore the relationship between protein sequence and aggregation, with a focus on biopharmaceuticals. She joined us having completed her BSc at the University of Newcastle.
Mr Alex Page
Joining the group in 2019 from The University of Sheffield and funded by a BBSRC CTP with Astra Zeneca, Alex is investigating how flow causes proteins to misfold and aggregate. Combining flow mechanics with fluorescence and other biophysical techniques his project aims to delineate an atomic model for flow-induced protein misfolding. He is jointly supervised with Dr David Brockwell.
Ms Ioanna Panagi|
Ioanna is a PhD student funded by the BBSRC working with Nik Kapur in the School of Mechanical Engineering, Ioanna is bringing her skills in fluid dynamics to the group. Her project is also co-supervised by Dr David Brockwell and will build novel devices and surfaces to help elucidate how, and why, flow causes proteins to aggregate. She joined the group in October 2017 having completed a BSc in Mechanical Engineering at the University of Leeds.
Dr Leon Willis|
Leon joined the 'Biologics' team in 2014 when he commenced his EPSRC-funded PhD supervised jointly with David Brockwell and Nic Kapur. Leon currently holds an EPSRC doctoral Fellowship and is co-funded by AstraZeneca. He is continuing his fundamental studies of how and why flow causes proteins to aggregate
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(people who started in the group between)
We would like to thank the following organisations for the funds to support our research:
BBSRC, MRC, EPSRC, The Royal Society, The Wellcome Trust, and The University of Leeds.