Publications

  1. A potent sybody selectively inhibits α-synuclein amyloid formation by binding to the p1 region. {Gialama, Dimitra , Vadukul, Devkee M. , Thrush, Rebecca J. , Radford, Sheena E. and Aprile, Francesco A.} (2024) Journal of Medicinal Chemistry, 67, 9857-9868. doi:10.1021/acs.jmedchem.3c02408
  2. CryoET of β-amyloid and tau within postmortem Alzheimer's disease brain. Gilbert, Madeleine A. G. , Fatima, Nayab , Jenkins, Joshua , O'Sullivan, Thomas J. , Schertel, Andreas , Halfon, Yehuda , Wilkinson, Martin , Morrema, Tjado H. J. , Geibel, Mirjam , Read, R,y J. , Ranson, Neil A. , Radford, Sheena E. , Hoozemans, Jeroen J. M. and Frank, Rene A. W. (2024) Nature, 631, 913-919. doi:10.1038/s41586-024-07680-x
  3. Single molecule delivery into living cells. Chau, Chalmers C. , Maffeo, Christopher M. , Aksimentiev, Aleksei , Radford, Sheena E. , Hewitt, Eric W. and Actis, Paolo (2024) Nature Communications, 15, 4403. doi:10.1038/s41467-024-48608-3
  4. Substitution of Met-38 to Ile in γ- synuclein found in two patients with amyotrophic lateral sclerosis induces aggregation into amyloid. Aubrey, Liam D. , Ninkina, Natalia , Ulamec, Sabine M. , Abramycheva, Natalia Y. , Vasili, Eftychia , Devine, Oliver M. , Wilkinson, Martin , Mackinnon, Eilish , Limorenko, Galina , Walko, Martin , Muwanga, Sarah , Amadio, Leonardo , Peters, Owen M. , Illarioshkin, Sergey N. , Outeiro, Tiago F. , Ranson, Neil A. , Brockwell, David J. , Buchman, Vladimir L. and Radford, Sheena E. (2024) Proceedings of the National Academy of Sciences of the United States of America, 121, . doi:10.1073/pnas.2309700120
  5. Local structural preferences in shaping tau amyloid polymorphism. Louros, Nikolaos , Wilkinson, Martin , Tsaka, Grigoria , Ramakers, Meine , Morelli, Chiara , Garcia, Teresa , Gallardo, Rodrigo , D'Haeyer, Sam , Goossens, Vera , Audenaert, Dominique , Thal, Dietmar Rudolf , Mackenzie, Ian R. , Rademakers, Rosa , Ranson, Neil A. , Radford, Sheena E. , Rousseau, Frederic and Schymkowitz, Joost (2024) Nature Communications, 15, 1028. doi:10.1038/s41467-024-45429-2
  6. A targetable N-terminal motif orchestrates α-synuclein oligomer-to-fibril conversion. Santos, Jaime , Cuellar, Jorge , Pallares, Irantzu , Byrd, Emily J. , Lends, Alons , Moro, Fern,o , Abdul-Shukkoor, Muhammed Bilal , Pujols, Jordi , Velasco-Carneros, Lorea , Sobott, Frank , Otzen, Daniel E. , Calabrese, Antonio N. , Muga, Arturo , Pedersen, Jan Skov , Loquet, Antoine , Valpuesta, Jose Maria , Radford, Sheena E. and Ventura, Salvador (2024) Journal of the American Chemical Society, 146, 12702-12711. doi:10.1021/jacs.4c02262
  7. Outer membrane protein assembly mediated by BAM-SurA complexes. Fenn, Katherine L. , Horne, Jim E. , Crossley, Joel A. , Bohringer, Nils , Horne, Romany J. , Schaberle, Till F. , Calabrese, Antonio N. , Radford, Sheena E. and Ranson, Neil A. (2024) Nature Communications, 15, 7612. doi:10.1038/s41467-024-51358-x
  8. Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec translocon. Crossley, Joel A. , Allen, William J. , Watkins, Daniel W. , Sabir, Tara , Radford, Sheena E. , Tuma, Roman , Collinson, Ian and Fessl, Tomas (2024) Embo Journal, 43, 1-13. doi:10.1038/s44318-023-00004-1
  9. Exploring a role for flow-induced aggregation assays in platform formulation optimisation for antibody-based proteins. Willis, Leon F. , Toprani, Vishal , Wijetunge, Sashini , Sievers, Annette , Lin, Laura , Williams, Jeanine , Crowley, Tom J. , Radford, Sheena E. , Kapur, Nikil and Brockwell, David J. (2024) Journal of Pharmaceutical Sciences, 113, 625-636. doi:10.1016/j.xphs.2023.10.031
  10. Protein-lipid charge interactions control the folding of outer membrane proteins into asymmetric membranes. Machin, Jonathan M. , Kalli, Antreas C. , Ranson, Neil A. and Radford, Sheena E. (2023) Nature Chemistry, 15, 1754-1764. doi:10.1038/s41557-023-01319-6
  11. Structural evolution of fibril polymorphs during amyloid assembly. Wilkinson, Martin , Xu, Yong , Thacker, Dev , Fisher, Declan G. , Taylor, Alexander I. P. , Gallardo, Rodrigo U. , Radford, Sheena E. and Ranson, Neil A. (2023) Cell, 186, P5798-5811. doi:10.1016/j.cell.2023.11.025
  12. Mechanistic study of the conductance and enhanced single-molecule detection in a polymer-electrolyte nanopore. Marcuccio, Fabio , Soulias, Dimitrios , Chau, Chalmers C. C. , Radford, Sheena E. , Hewitt, Eric , Actis, Paolo and Edwards, Martin Andrew (2023) ACS Nanoscience Au, 3, 172-181. doi:10.1021/acsnanoscienceau.2c00050
  13. Disease-relevant β2-microglobulin variants share a common amyloid fold. Wilkinson, Martin , Gallardo, Rodrigo U. , Martinez, Roberto Maya , Guthertz, Nicolas , So, Masatomo , Aubrey, Liam D. , Radford, Sheena E. and Ranson, Neil A. (2023) Nature Communications, 14, 1190. doi:10.1038/s41467-023-36791-8
  14. Changing shape of molecules. Radford, Sheena E. and Summers, Michael F. (2023) Journal of Molecular Biology, 435, . doi:10.1016/j.jmb.2023.168092
  15. The in-tissue molecular architecture of beta-amyloid pathology in the mammalian brain. Leistner, Conny , Wilkinson, Martin , Burgess, Ailidh , Lovatt, Megan , Goodbody, Stanley , Xu, Yong , Deuchars, Susan , Radford, Sheena E. , Ranson, Neil A. and Frank, Rene A. W. (2023) Nature Communications, 14, 2833. doi:10.1038/s41467-023-38495-5
  16. Single-molecule fluorescence: elucidating ATP driven protein transport in the Sec complex. Crossley, Joel A. , Fessl, Tomas , Collinson, Ian , Tuma, Roman and Radford, Sheena E. (2023) Biophysical Journal, 122, 50A. doi:
  17. The delivery complex in outer membrane protein biogenesis: how the SurA chaperone interacts with the BAM enzyme. Horne, Jim E. , Fenn, Katherine , Crossley, Joel A. , Radford, Sheena E. and Ranson, Neil A. (2023) Biophysical Journal, 122, 343A. doi:
  18. Darobactin B stabilises a lateral-closed conformation of the BAM complex in E. coli cells. Haysom, Samuel F. , Machin, Jonathan , Whitehouse, James M. , Horne, Jim E. , Fenn, Katherine , Ma, Yue , El Mkami, Hassane , Boehringer, Nils , Schaeberle, Till F. , Ranson, Neil A. , Radford, Sheena E. and Pliotas, Christos (2023) Angewandte Chemie-international Edition, , e202218783. doi:10.1002/anie.202218783
  19. Taking charge: metal ions accelerate amyloid aggregation in sequence variants of alpha-synuclein. Byrd, Emily J. , Wilkinson, Martin , Radford, Sheena E. and Sobott, Frank (2023) Journal of the American Society for Mass Spectrometry, 34, 493-504. doi:10.1021/jasms.2c00379
  20. Controlling amyloid formation of intrinsically disordered proteins and peptides: slowing down or speeding up?. Xu, Yong , Maya-Martinez, Roberto and Radford, Sheena E. (2022) Essays in Biochemistry, 66, 959-975. doi:10.1042/EBC20220046
  21. Dimers of D76N-beta 2-microglobulin display potent antiamyloid aggregation activity. Maya-Martinez, Roberto , Xu, Yong , Guthertz, Nicolas , Walko, Martin , Karamanos, Theodoros K. , Sobott, Frank , Breeze, Alexander L. and Radford, Sheena E. (2022) Journal of Biological Chemistry, 298, 102659. doi:10.1016/j.jbc.2022.102659
  22. Probing RNA conformations using a polymer-electrolyte solid-state nanopore. Chau, Chalmers , Marcuccio, Fabio , Soulias, Dimitrios , Edwards, Martin Andrew , Tuplin, Andrew , Radford, Sheena E. , Hewitt, Eric and Actis, Paolo (2022) ACS Nano, 16, 20075-20085. doi:10.1021/acsnano.2c08312
  23. Single residue modulators of amyloid formation in the N-terminal p1-region of alpha-synuclein. Ulamec, Sabine M. , Maya-Martinez, Roberto , Byrd, Emily J. , Dewison, Katherine M. , Xu, Yong , Willis, Leon F. , Sobott, Frank , Heath, George R. , Hawle, Patricija van Oosten , Buchman, Vladimir L. , Radford, Sheena E. and Brockwell, David J. (2022) Nature Communications, 13, 4986. doi:10.1038/s41467-022-32687-1
  24. Sequence grammar underlying the unfolding and phase separation of globular proteins. Ruff, Kiersten M. , Choi, Yoon Hee , Cox, Dezerae , Ormsby, Angelique R. , Myung, Yoochan , Ascher, David B. , Radford, Sheena E. , V. Pappu, Rohit and Hatters, Danny M. (2022) Molecular Cell, 82, 3193-3208. doi:10.1016/j.molcel.2022.06.024
  25. The effect of mutation on an aggregation-prone protein: an in vivo, in vitro, and in silico analysis. Guthertz, N. , van Der Kant, R. , Martinez, R. M. , Xu, Y. , Trinh, C. , Iorga, B. I. , Rousseau, F. , Schymkowitz, J. , Brockwell, D. J. and Radford, S. E. (2022) Proceedings of the National Academy of Sciences of the United States of America, 119, e2200468119. doi:10.1073/pnas.2200468119
  26. Trigger factor both holds and folds its client proteins. Wu, Kevin , Minshull, Thomas C. , Radford, Sheena E. , Calabrese, Antonio N. and Bardwell, James C. A. (2022) Nature Communications, 13, 4126. doi:10.1038/s41467-022-31767-6
  27. Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding. Schiffrin, Bob , Machin, Jonathan M. , Karamanos, Theodoros K. , Zhuravleva, Anastasia , Brockwell, David J. , Radford, Sheena E. and Calabrese, Antonio N. (2022) Communications Biology, 5, 560. doi:10.1038/s42003-022-03502-w
  28. High-throughput directed evolution: a golden era for protein science. McLure, Romany J. , Radford, Sheena E. and Brockwell, David J. (2022) Trends in Chemistry, 4, 378-391. doi:10.1016/j.trechm.2022.02.004
  29. Generating ensembles of dynamic misfolding proteins. Karamanos, Theodoros K. , Kalverda, Arnout P. and Radford, Sheena E. (2022) Frontiers in Neuroscience, 16, . doi:10.3389/fnins.2022.881534
  30. Roll out the barrel! Outer membrane tension resolves an unexpected folding intermediate. Horne, Jim E. and Radford, Sheena E. (2022) Cell, 185, 1107-1109. doi:10.1016/j.cell.2022.03.001
  31. Tuning the rate of aggregation of hiapp into amyloid using small-molecule modulators of assembly. Xu, Yong, Maya-Martinez, Roberto, Guthertz, Nicolas, Heath, George R., Manfield, Iain W., Breeze, Alexander L., Sobott, Frank, Foster, Richard and Radford, Sheena E. (2022) Nature Communications, 13, 1040. doi:10.1038/s41467-022-28660-7
  32. Global proteotoxicity caused by human beta(2) microglobulin variants impairs the unfolded protein response in C. elegans. Good, Sarah C. , Dewison, Katherine M. , Radford, Sheena E. and van Oosten-Hawle, Patricija (2021) international Journal of Molecular Sciences, 22, 10752. doi:10.3390/ijms221910752
  33. Alphafold: a special issue and a special time for protein science. Serpell, Louise C. , Radford, Sheena E. and Otzen, Daniel E. (2021) Journal of Molecular Biology, 433, . doi:
  34. The role of membrane destabilisation and protein dynamics in bam catalysed omp folding. White, Paul , Haysom, Samuel F. , Iadanza, Matthew G. , Higgins, Anna J. , Machin, Jonathan M. , Whitehouse, James M. , Horne, Jim E. , Schiffrin, Bob , Carpenter-Platt, Charlotte , Calabrese, Antonio N. , Storek, Kelly M. , Rutherford, Steven T. , Brockwell, David J. , Ranson, Neil A. and Radford, Sheena E. (2021) Nature Communications, 12, 4174. doi:10.1038/s41467-021-24432-x
  35. Finding the sweet spot for chaperone activity. Radford, Sheena E. and Karamanos, Theodoros K. (2021) Nature Chemistry, 13, 397-399. doi:10.1038/s41557-021-00691-5
  36. Brazilin removes toxic alpha-synuclein and seeding competent assemblies from parkinson brain by altering conformational equilibrium. Nahass, George R. , Sun, Yuanzi , Xu, Yong , Batchelor, Mark , Reilly, Madeleine , Benilova, Iryna , Kedia, Niraja , Spehar, Kevin , Sobott, Frank , Sessions, Richard B. , Caughey, Byron , Radford, Sheena E. , Jat, Parmjit S. , Collinge, John and Bieschke, Jan (2021) Journal of Molecular Biology, 433, 166878. doi:10.1016/j.jmb.2021.166878
  37. De novo design of transmembrane beta barrels. Vorobieva, Anastassia A. , White, Paul , Liang, Binyong , Horne, Jim E. , Bera, Asim K. , Chow, Cameron M. , Gerben, Stacey , Marx, Sinduja , Kang, Alex , Stiving, Alyssa Q. , Harvey, Sophie R. , Marx, Dagan C. , Khan, G. Nasir , Fleming, Karen G. , Wysocki, Vicki H. , Brockwell, David J. , Tamm, Lukas K. , Radford, Sheena E. and Baker, David (2021) Science, 371, 801+. doi:10.1126/science.abc8182
  38. Proteostasis of islet amyloid polypeptide: a molecular perspective of risk factors and protective strategies for Type II diabetes. Milardi, Danilo , Gazit, Ehud , Radford, Sheena E. , Xu, Yong , Gallardo, Rodrigo U. , Caflisch, Amedeo , Westermark, Gunilla T. , Westermark, Per , La Rosa, Carmelo and Ramamoorthy, Ayyalusamy (2021) Chemical Reviews, 121, 1845-1893. doi:10.1021/acs.chemrev.0c00981
  39. Investigation of D76N beta(2)-microglobulin using protein footprinting and structural mass spectrometry. Cornwell, Owen , Ault, James R. , Bond, Nicholas J. , Radford, Sheena E. and Ashcroft, Alison E. (2021) Journal of the American Society for Mass Spectrometry, 32, 1583-1592. doi:10.1021/jasms.0c00438
  40. Visualizing and trapping transient oligomers in amyloid assembly pathways. Cawood, Emma E. , Karamanos, Theodoros K. , Wilson, Andrew J. and Radford, Sheena E. (2021) Biophysical Chemistry, 268, . doi:10.1016/j.bpc.2020.106505
  41. Modulation of amyloidogenic protein self-assembly using tethered small molecules. Cawood, Emma E. , Guthertz, Nicolas , Ebo, Jessica S. , Karamanos, Theodoros K. , Radford, Sheena E. and Wilson, Andrew J. (2020) Journal of the American Chemical Society, 142, 20845-20854. doi:10.1021/jacs.0c10629
  42. Distortion of the bilayer and dynamics of the BAM complex in lipid nanodiscs. Iadanza, Matthew G. , Schiffrin, Bob , White, Paul , Watson, Matthew A. , Horne, Jim E. , Higgins, Anna J. , Calabrese, Antonio N. , Brockwell, David J. , Tuma, Roman , Kalli, Antreas C. , Radford, Sheena E. and Ranson, Neil A. (2020) Communications Biology, 3, 766. doi:10.1038/s42003-020-01419-w
  43. Looking beyond the core: the role of flanking regions in the aggregation of amyloidogenic peptides and proteins. Ulamec, Sabine M. , Brockwell, David J. and Radford, Sheena E. (2020) Frontiers in Neuroscience, 14, . doi:10.3389/fnins.2020.611285
  44. Modulation of beta-amyloid fibril formation in Alzheimer's disease by microglia and infection. Brown, Madeleine R. , Radford, Sheena E. and Hewitt, Eric W. (2020) Frontiers in Molecular Neuroscience, 13, . doi:10.3389/fnmol.2020.609073
  45. Structural insights into peptide self-assembly using photo-induced crosslinking experiments and discontinuous molecular dynamics. Bunce, Samuel J. , Wang, Yiming , Radford, Sheena E. , Wilson, Andrew J. and Hall, Carol K. (2020) Aiche Journal, , . doi:10.1002/aic.17101
  46. The uniqueness of flow in probing the aggregation behavior of clinically relevant antibodies. Willis, Leon F. , Kumar, Amit , Jain, Tushar , Caffrey, Isabelle , Xu, Yingda , Radford, Sheena E. , Kapur, Nikil , Vasquez, Maximiliano and Brockwell, David J. (2020) Engineering Reports, 27, 1094. doi:10.1002/eng2.12147
  47. Fibril structures of diabetes-related amylin variants reveal a basis for surface-templated assembly. Gallardo, Rodrigo , Iadanza, Matthew G. , Xu, Yong , Heath, George R. , Foster, Richard , Radford, Sheena E. and Ranson, Neil A. (2020) Nature Structural & Molecular Biology, 27, 1094. doi:10.1038/s41594-020-00525-x, Early Access Date = OCT 2020
  48. Potential human transmission of amyloid beta pathology: surveillance and risks. Lauwers, Elsa , Lalli, Giovanna , Br,ner, Sebastian , Collinge, John , Compernolle, Veerle , Duyckaerts, Charles , Edgren, Gustaf , Haik, Stephane , Hardy, John , Helmy, Adel , Ivinson, Adrian J. , Jaunmuktane, Zane , Jucker, Mathias , Knight, Richard , Lemmens, Robin , Lin, I-Chun , Love, Seth , Mead, Simon , Perry, V. Hugh , Pickett, James , Poppy, Guy , Radford, Sheena E. , Rousseau, Frederic , Routledge, Carol , Schiavo, Giampietro , Schymkowitz, Joost , Selkoe, Dennis J. , Smith, Colin , Thal, Dietmar R. , Theys, Tom , Tiberghien, Pierre , van den Burg, Peter , V,ekerckhove, Philippe , Walton, Clare , Zaaijer, Hans L. , Zetterberg, Henrik and De Strooper, Bart (2020) Lancet Neurology, 19, 872-878. doi:
  49. The role of the IT-state in D76N beta(2)-microglobulin amyloid assembly: a crucial intermediate or an innocuous bystander?. Smith, I, Hugh , Guthertz, Nicolas , Cawood, Emma E. , Maya-Martinez, Roberto , Breeze, Alexander L. and Radford, Sheena E. (2020) Journal of Biological Chemistry, 295, 12474-12484. doi:10.1074/jbc.RA120.014901
  50. Spot the difference: function versus toxicity in amyloid fibrils. Ulamec, Sabine M. and Radford, Sheena E. (2020) Trends in Biochemical Sciences, 45, 635-636. doi:10.1016/j.tibs.2020.04.007
  51. Role of the lipid bilayer in outer membrane protein folding in gram-negative bacteria. Horne, Jim E. , Brockwell, David J. and Radford, Sheena E. (2020) Journal of Biological Chemistry, 295, 10340-10367. doi:10.1074/jbc.REV120.011473
  52. Macromolecular crowding enhances the detection of DNA and proteins by a solid-state nanopore. Chau, Chalmers C. , Radford, Sheena E. , Hewitt, Eric W. and Actis, Paolo (2020) Nano Letters, 20, 5553-5561. doi:10.1021/acs.nanolett.0c02246
  53. Pyxlinkviewer: a flexible tool for visualization of protein chemical crosslinking data within thepymolmolecular graphics system. Schiffrin, Bob , Radford, Sheena E. , Brockwell, David J. and Calabrese, Antonio N. (2020) Protein Science, 29, 1851-1857. doi:10.1002/pro.3902, Early Access Date = JUL 2020
  54. Structural insight into the formation of lipoprotein-beta-barrel complexes. Rodriguez-Alonso, Raquel , Letoquart, Juliette , Van Son Nguyen , Louis, Gwennaelle , Calabrese, Antonio N. , Iorga, Bogdan I. , Radford, Sheena E. , Cho, Seung-Hyun , Remaut, Han and Collet, Jean-Francois () Nature Chemical Biology, , . doi:10.1038/s41589-020-0575-0, Early Access Date = JUN 2020
  55. Inter-domain dynamics in the chaperone SurA and multi-site binding to its outer membrane protein clients. Calabrese, Antonio N. , Schiffrin, Bob , Watson, Matthew , Karamanos, Theodoros K. , Walko, Martin , Humes, Julia R. , Horne, Jim E. , White, Paul , Wilson, Andrew J. , Kalli, Antreas C. , Tuma, Roman , Ashcroft, Alison E. , Brockwell, David J. and Radford, Sheena E. (2020) Nature Communications, 11, 2155. doi:10.1038/s41467-020-15702-1
  56. An in vivo platform to select and evolve aggregation-resistant proteins. Ebo, Jessica S. , Saunders, Janet C. , Devine, Paul W. A. , Gordon, Alice M. , Warwick, Amy S. , Schiffrin, Bob , Chin, Stacey E. , Engl,, Elizabeth , Button, James D. , Lloyd, Christopher , Bond, Nicholas J. , Ashcroft, Alison E. , Radford, Sheena E. , Lowe, David C. and Brockwell, David J. (2020) Nature Communications, 11, 1816. doi:10.1038/s41467-020-15667-1
  57. A short motif in the N-terminal region of alpha-synuclein is critical for both aggregation and function. Doherty, Ciaran P. A. , Ulamec, Sabine M. , Maya-Martinez, Roberto , Good, Sarah C. , Makepeace, Jemma , Khan, G. Nasir , van Oosten-Hawle, Patricija , Radford, Sheena E. and Brockwell, David J. (2020) Nature Structural & Molecular Biology, 27, 249-259. doi:10.1038/s41594-020-0384-x
  58. Amyloid structures: much more than just a cross-beta fold. Gallardo, Rodrigo , Ranson, Neil A. and Radford, Sheena E. (2020) Current Opinion in Structural Biology, 60, 7-16. doi:10.1016/j.sbi.2019.09.001
  59. Using protein engineering to understand and modulate aggregation. Ebo, Jessica S. , Guthertz, Nicolas , Radford, Sheena E. and Brockwell, David J. (2020) Current Opinion in Structural Biology, 60, 157-166. doi:10.1016/j.sbi.2020.01.005
  60. Collagen I weakly interacts with the beta-sheets of beta(2)-microglobulin and enhances conformational exchange to induce amyloid formation. Hoop, Cody L. , Zhu, Jie , Bhattacharya, Shibani , Tobita, Caitlyn A. , Radford, Sheena E. and Baum, Jean (2020) Journal of the American Chemical Society, 142, 1321-1331. doi:10.1021/jacs.9b10421
  61. Homage to Chris Dobson. Baum, Jean , Chiti, Fabrizio , De Simone, Alfonso , Knowles, Tuomas P. J. , Kumita, Janet R. , Radford, Sheena E. , Robinson, Carol V. , Salvatella, Xavier , Valelli, Karen , Vendruscolo, Michele , Pastore, Annalisa and Tartaglia, Gian Gaetano (2019) Frontiers in Molecular Biosciences, 6, . doi:10.3389/fmolb.2019.00137
  62. Long-range conformational changes in monoclonal antibodies revealed using FPOP-LS-MS/MS. Cornwell, Owen , Bond, Nicholas J. , Radford, Sheena E. and Ashcroft, Alison E. (2019) Analytical Chemistry, 91, 15163-15170. doi:10.1021/acs.analchem.9b03958
  63. Structural mapping of oligomeric intermediates in an amyloid assembly pathway. Karamanos, Theodoros K. , Jackson, Matthew P. , Calabrese, Antonio N. , Goodchild, Sophia C. , Cawood, Emma E. , Section, Gary S. Thompson , Kalverda, Arnout P. , Hewitt, Eric W. and Radford, Sheena E. (2019) Elife, 8, . doi:10.7554/eLife.46574
  64. Extracellular matrix components modulate different stages in (2)-microglobulin amyloid formation. Benseny-Cases, Nuria , Karamanos, Theodoros K. , Hoop, Cody L. , Baum, Jean and Radford, Sheena E. (2019) Journal of Biological Chemistry, 294, 9392-9401. doi:10.1074/jbc.RA119.008300
  65. Molecular insights into the surface-catalyzed secondary nucleation of amyloid-beta(40) (a beta(40)) by the peptide fragment a beta(16-22). Bunce, Samuel J. , Wang, Yiming , Stewart, Katie L. , Ashcroft, Alison E. , Radford, Sheena E. , Hall, Carol K. and Wilson, Andrew J. (2019) Science Advances, 5, . doi:10.1126/sciadv.aav8216
  66. Dual role of ribosome-binding domain of nac as a potent suppressor of protein aggregation and aging-related proteinopathies. Shen, Koning , Gamerdinger, Martin , Chan, Rebecca , Gense, Karina , Martin, Esther M. , Sachs, Nadine , Knight, Patrick D. , Schloemer, Renate , Calabrese, Antonio N. , Stewart, Katie L. , Leiendecker, Lukas , Baghel, Ankit , Radford, Sheena E. , Frydman, Judith and Deuerling, Elke (2019) Molecular Cell, 74, 729-741. doi:10.1016/j.molcel.2019.03.012
  67. The role of SurA pplase domains in preventing aggregation of the outer-membrane proteins tOmpA and OmpT. Humes, Julia R. , Schiffrin, Bob , Calabrese, Antonio N. , Higgins, Anna J. , Westhead, David R. , Brockwell, David J. and Radford, Sheena E. (2019) Journal of Molecular Biology, 431, 1267-1283. doi:10.1016/j.jmb.2019.01.032
  68. Thermodynamic phase diagram of amyloid-beta (16-22) peptide. Wang, Yiming , Bunce, Samuel J. , Radford, Sheena E. , Wilson, Andrew J. , Auer, Stefan and Hall, Carol K. (2019) Proceedings of the National Academy of Sciences of the United States of America, 116, 2091-2096. doi:10.1073/pnas.1819592116
  69. Design and synthesis of cysteine-specific labels for photo-crosslinking studies. Walko, Martin , Hewitt, Eric , Radford, Sheena E. and Wilson, Andrew J. (2019) Rsc Advances, 9, 7610-7614. doi:10.1039/c8ra10436k
  70. Rapid mapping of protein interactions using tag-transfer photocrosslinkers. Horne, Jim E. , Walko, Martin , Calabrese, Antonio N. , Levenstein, Mark A. , Brockwell, David J. , Kapur, Nikil , Wilson, Andrew J. and Radford, Sheena E. (2018) Angewandte Chemie-international Edition, 57, 16688-16692. doi:10.1002/anie.201809149
  71. A new era for understanding amyloid structures and disease. Iadanza, Matthew G. , Jackson, Matthew P. , Hewitt, Eric W. , Ranson, Neil A. and Radford, Sheena E. (2018) Nature Reviews Molecular Cell Biology, 19, 755-773. doi:10.1038/s41580-018-0060-8
  72. Comparing hydrogen deuterium exchange and fast photochemical oxidation of proteins: a structural characterisation of wild-type and Delta N6 beta(2)-microglobulin. Cornwell, Owen , Radford, Sheena E. , Ashcroft, Alison E. and Ault, James R. (2018) Journal of the American Society for Mass Spectrometry, 29, 2413-2426. doi:10.1007/s13361-018-2067-y
  73. Orientation of a diagnostic ligand bound to macroscopically aligned amyloid-beta fibrils determined by solid-state NMR. Townsend, David , Hughes, Eleri , Stewart, Katie L. , Griffin, John M. , Radford, Sheena E. and Middleton, David A. (2018) Journal of Physical Chemistry Letters, 9, 6611-6615. doi:10.1021/acs.jpclett.8b02448
  74. The structure of a beta(2)-microglobulin fibril suggests a molecular basis for its amyloid polymorphism. Iadanza, Matthew G. , Silvers, Robert , Boardman, Joshua , Smith, Hugh I. , Karamanos, Theodoros K. , Debelouchina, Galia T. , Su, Yongchao , Griffin, Robert G. , Ranson, Neil A. and Radford, Sheena E. (2018) Nature Communications, 9, 4517. doi:10.1038/s41467-018-06761-6
  75. Mass spectrometry-enabled structural biology of membrane proteins. Calabrese, Antonio N. and Radford, Sheena E. (2018) Methods, 147, 187-205. doi:10.1016/j.ymeth.2018.02.020
  76. Epigallocatechin-3-gallate remodels apolipoprotein a-i amyloid fibrils into soluble oligomers in the presence of heparin. Townsend, David , Hughes, Eleri , Akien, Geoffrey , Stewart, Katie L. , Radford, Sheena E. , Rochester, David and Middleton, David A. (2018) Journal of Biological Chemistry, 293, 12877-12893. doi:10.1074/jbc.RA118.002038
  77. A peptide-display protein scaffold to facilitate single molecule force studies of aggregation-prone peptides. Doherty, Ciaran P. A. , Young, Lydia M. , Karamanos, Theodoros K. , Smith, Hugh I. , Jackson, Matthew P. , Radford, Sheena E. and Brockwell, David J. (2018) Protein Science, 27, 1205-1217. doi:10.1002/pro.3386
  78. The role of beta-barrels 1 and 2 in the enzymatic activity of factor xiii a-subunit. Hethershaw, E. L. , Adamson, P. J. , Smith, K. A. , Goldsberry, W. N. , Pease, R. J. , Radford, S. E. , Grant, P. J. , Ariens, R. A. S. , Maurer, M. C. and Philippou, H. (2018) Journal of Thrombosis and Haemostasis, 16, 1391-1401. doi:10.1111/jth.14128
  79. Dynamic action of the sec machinery during initiation, protein translocation and termination. Fessl, Tomas , Watkins, Daniel , Oatley, Peter , Allen, William John , Corey, Robin Adam , Horne, Jim , Baldwin, Steve A. , Radford, Sheena E. , Collinson, Ian and Tuma, Roman (2018) Elife, 7, . doi:10.7554/eLife.35112
  80. Conformational flexibility within the nascent polypeptide-associated complex enables its interactions with structurally diverse client proteins. Martin, Esther M. , Jackson, Matthew P. , Gamerdinger, Martin , Gense, Karina , Karamonos, Theodoros K. , Humes, Julia R. , Deuerling, Elke , Ashcroft, Alison E. and Radford, Sheena E. (2018) Journal of Biological Chemistry, 293, 8554-8568. doi:10.1074/jbc.RA117.001568
  81. Using extensional flow to reveal diverse aggregation landscapes for three IgG1 molecules. Willis, Leon F. , Kumar, Amit , Dobson, John , Bond, Nicholas J. , Lowe, David , Turner, Richard , Radford, Sheena E. , Kapur, Nikil and Brockwell, David J. (2018) Biotechnology and Bioengineering, 115, 1216-1225. doi:10.1002/bit.26543
  82. Identification of a novel site of interaction between ataxin-3 and the amyloid aggregation inhibitor polyglutamine binding peptide 1. Knight, Patrick D. , Karamanos, Theodoros K. , Radford, Sheena E. and Ashcroft, Alison E. (2018) European Journal of Mass Spectrometry, 24, 129-140. doi:10.1177/1469066717729298
  83. Outer membrane protein folding from an energy landscape perspective. Schiffrin, Bob , Brockwell, David J. and Radford, Sheena E. (2017) Bmc Biology, 15, . doi:10.1186/s12915-017-0464-5
  84. Effects of periplasmic chaperones and membrane thickness on bama-catalyzed outer-membrane protein folding. Schiffrin, Bob , Calabrese, Antonio N. , Higgins, Anna J. , Humes, Julia R. , Ashcroft, Alison E. , Kalli, Antreas C. , Brockwell, David J. and Radford, Sheena E. (2017) Journal of Molecular Biology, 429, 3776-3792. doi:10.1016/j.jmb.2017.09.008
  85. Topological dissection of the membrane transport protein mhp1 derived from cysteine accessibility and mass spectrometry. Calabrese, Antonio N. , Jackson, Scott M. , Jones, Lynsey N. , Beckstein, Oliver , Heinkel, Florian , Gsponer, Joerg , Sharples, David , Sans, Marta , Kokkinidou, Maria , Pearson, Arwen R. , Radford, Sheena E. , Ashcroft, Alison E. and Henderson, Peter J. F. (2017) Analytical Chemistry, 89, 8844-8852. doi:10.1021/acs.analchem.7b01310
  86. Investigating the structural compaction of biomolecules upon transition to the gas-phase using esi-twims-ms. Devine, Paul W. A. , Fisher, Henry C. , Calabrese, Antonio N. , Whelan, Fiona , Higazi, Daniel R. , Potts, Jennifer R. , Lowe, David C. , Radford, Sheena E. and Ashcroft, Alison E. (2017) Journal of the American Society for Mass Spectrometry, 28, 1855-1862. doi:10.1007/s13361-017-1689-9
  87. Molecular origins of the compatibility between glycosaminoglycans and a beta 40 amyloid fibrils. Stewart, Katie L. , Hughes, Eleri , Yates, Edwin A. , Middleton, David A. and Radford, Sheena E. (2017) Journal of Molecular Biology, 429, 2449-2462. doi:10.1016/j.jmb.2017.07.003
  88. Small molecule probes of protein aggregation. Young, Lydia M. , Ashcroft, Alison E. and Radford, Sheena E. (2017) Current Opinion in Chemical Biology, 39, 90-99. doi:10.1016/j.cbpa.2017.06.008
  89. Lateral opening in the intact beta-barrel assembly machinery captured by cryo-EM. Higgins, A. J. , Iadanza, M. G. , Schiffrin, B. , Calabrese, A. N. , Humes, J. R. , Brockwell, D. J. , Ashcroft, A. E. , Ranson, N. A. and Radford, S. E. (2017) European Biophysics Journal With Biophysics Letters, 46, S346. doi:
  90. Complementary cross-linking and fluorescence studies of outer membrane protein biogenesis in vitro. Horne, J. E. , Radford, S. E. and Brockwell, D. J. (2017) European Biophysics Journal With Biophysics Letters, 46, S346. doi:
  91. Identifying the contributions of the different domains within SurA on outer member protein folding. Humes, J. R. , Higgins, A. J. , Brockwell, D. J. and Radford, S. E. (2017) European Biophysics Journal With Biophysics Letters, 46, S346. doi:
  92. The challenges and opportunities of understanding protein folding and misfolding in health and disease. Radford, S. E. (2017) European Biophysics Journal With Biophysics Letters, 46, S44. doi:
  93. Understanding co-polymerization in amyloid formation by direct observation of mixed oligomers. Young, Lydia M. , Tu, Ling-Hsien , Raleigh, Daniel P. , Ashcroft, Alison E. and Radford, Sheena E. (2017) Chemical Science, 8, 5030-5040. doi:10.1039/c7sc00620a
  94. Inducing protein aggregation by extensional flow. Dobson, John , Kumar, Amit , Willis, Leon F. , Tuma, Roman , Higazi, Daniel R. , Turner, Richard , Lowe, David C. , Ashcroft, Alison E. , Radford, Sheena E. , Kapur, Nikil and Brockwell, David J. (2017) Proceedings of the National Academy of Sciences of the United States of America, 114, 4673-4678. doi:10.1073/pnas.1702724114
  95. Fretting about outer membrane protein biogenesis. How exactly do they fold?. Horne, Jim E. , Brockwell, David J. and Radford, Sheena E. (2017) Biophysical Journal, 112, 205A. doi:10.1016/j.bpj.2016.11.1131
  96. FPOP-LC-MS-MS suggests differences in interaction sites of amphipols and detergents with outer membrane proteins. Watkinson, Thomas G. , Calabrese, Antonio N. , Ault, James R. , Radford, Sheena E. and Ashcroft, Alison E. (2017) Journal of the American Society for Mass Spectrometry, 28, 50-55. doi:10.1007/s13361-016-1421-1
  97. Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo. Dobson, Claire L. , Devine, Paul W. A. , Phillips, Jonathan J. , Higazi, Daniel R. , Lloyd, Christopher , Popovic, Bojana , Arnold, Joanne , Buchanan, Andrew , Lewis, Arthur , Goodman, Joanne , van der Walle, Christopher F. , Thornton, Peter , Vinall, Lisa , Lowne, David , Aagaard, Anna , Olsson, Lise-Lotte , Wollberg, Anna Ridderstad , Welsh, Fraser , Karamanos, Theodoros K. , Pashley, Clare L. , Iadanza, Matthew G. , Ranson, Neil A. , Ashcroft, Alison E. , Kippen, Alistair D. , Vaughan, Tristan J. , Radford, Sheena E. and Lowe, David C. (2016) Scientific Reports, 6, . doi:10.1038/srep38644
  98. Lateral opening in the intact beta-barrel assembly machinery captured by cryo-EM. Iadanza, Matthew G. , Higgins, Anna J. , Schiffrin, Bob , Calabrese, Antonio N. , Brockwell, David J. , Ashcroft, Alison E. , Radford, Sheena E. and Ranson, Neil A. (2016) Nature Communications, 7, . doi:10.1038/ncomms12865
  99. Skp is a multivalent chaperone of outer-membrane proteins. Schiffrin, Bob , Calabrese, Antonio N. , Devine, Paul W. A. , Harris, Sarah A. , Ashcroft, Alison E. , Brockwell, David J. and Radford, Sheena E. (2016) Nature Structural & Molecular Biology, 23, 786-793. doi:10.1038/nsmb.3266
  100. Atomic details of the interactions of glycosaminoglycans with amyloid-beta fibrils. Stewart, Katie L. , Hughes, Eleri , Yates, Edwin A. , Akien, Geoffrey R. , Huang, Teng-Yi , Lima, Marcelo A. , Rudd, Timothy R. , Guerrini, Marco , Hung, Shang-Cheng , Radford, Sheena E. and Middleton, David A. (2016) Journal of the American Chemical Society, 138, 8328-8331. doi:10.1021/jacs.6b02816
  101. A growing toolbox of techniques for studying beta-barrel outer membrane protein folding and biogenesis. Horne, Jim E. and Radford, Sheena E. (2016) Biochemical Society Transactions, 44, 802-809. doi:10.1042/BST20160020
  102. A population shift between sparsely populated folding intermediates determines amyloidogenicity. Karamanos, Theodoros K. , Pashley, Clare L. , Kalverda, Arnout P. , Thompson, Gary S. , Mayzel, Maxim , Orekhov, Vladislav Y. and Radford, Sheena E. (2016) Journal of the American Chemical Society, 138, 6271-6280. doi:10.1021/jacs.6b02464
  103. Two-way communication between secy and seca suggests a brownian ratchet mechanism for protein translocation. Allen, William John , Corey, Robin Adam , Oatley, Peter , Sessions, Richard Barry , Baldwin, Steve A. , Radford, Sheena E. , Tuma, Roman and Collinson, Ian (2016) Elife, 5, . doi:10.7554/eLife.15598
  104. Esi-ims-ms: a method for rapid analysis of protein aggregation and its inhibition by small molecules. Young, Lydia M. , Saunders, Janet C. , Mahood, Rachel A. , Revill, Charlotte H. , Foster, Richard J. , Ashcroft, Alison E. and Radford, Sheena E. (2016) Methods, 95, 62-69. doi:10.1016/j.ymeth.2015.05.017
  105. Comparison of the aggregation of homologous beta(2)-microglobulin variants reveals protein solubility as a key determinant of amyloid formation. Pashley, Clare L. , Hewitt, Eric W. and Radford, Sheena E. (2016) Journal of Molecular Biology, 428, 631-643. doi:10.1016/j.jmb.2016.01.009
  106. Mpul-multi: software for calculation of amyloid fibril mass per unit length from tb-tem images. Iadanza, Matthew G. , Jackson, Matthew P. , Radford, Sheena E. and Ranson, Neil A. (2016) Scientific Reports, 6, . doi:10.1038/srep21078
  107. An in vivo platform for identifying inhibitors of protein aggregation. Saunders, Janet C. , Young, Lydia M. , Mahood, Rachel A. , Jackson, Matthew P. , Revill, Charlotte H. , Foster, Richard J. , Smith, D. Alastair , Ashcroft, Alison E. , Brockwell, David J. and Radford, Sheena E. (2016) Nature Chemical Biology, 12, 94+. doi:10.1038/NCHEMBIO.1988
  108. Substrate protein folds while it is bound to the ATP-independent chaperone spy. Stull, Frederick , Koldewey, Philipp , Humes, Julia R. , Radford, Sheena E. and Bardwell, James C. A. (2016) Nature Structural & Molecular Biology, 23, 53-58. doi:10.1038/nsmb.3133
  109. Amyloid fibres: inert end-stage aggregates or key players in disease?. Tipping, Kevin W. , van Oosten-Hawle, Patricija , Hewitt, Eric W. and Radford, Sheena E. (2015) Trends in Biochemical Sciences, 40, 719-727. doi:10.1016/j.tibs.2015.10.002
  110. Systematic analysis of the use of amphipathic polymers for studies of outer membrane proteins using mass spectrometry. Watkinson, Thomas G. , Calabrese, Antonio N. , Giusti, Fabrice , Zoonens, Manuela , Radford, Sheena E. and Ashcroft, Alison E. (2015) international Journal of Mass Spectrometry, 391, 54-61. doi:10.1016/j.ijms.2015.06.017
  111. Mass spectrometric methods to analyze the structural organization of macromolecular complexes. Rajabi, Khadijeh , Ashcroft, Alison E. and Radford, Sheena E. (2015) Methods, 89, 13-21. doi:10.1016/j.ymeth.2015.03.004
  112. Using hydroxyl radical footprinting to explore the free energy landscape of protein folding. Calabrese, Antonio N. , Ault, James R. , Radford, Sheena E. and Ashcroft, Alison E. (2015) Methods, 89, 38-44. doi:10.1016/j.ymeth.2015.02.018
  113. Mechanisms of amyloid formation revealed by solution NMR. Karamanos, Theodoros K. , Kalverda, Arnout P. , Thompson, Gary S. and Radford, Sheena E. (2015) Progress in Nuclear Magnetic Resonance Spectroscopy, 88-89, 86-104. doi:10.1016/j.pnmrs.2015.05.002
  114. A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry. Rajabi, Khadijeh , Reuther, Julia , Deuerling, Elke , Radford, Sheena E. and Ashcroft, Alison E. (2015) Protein Science, 24, 1282-1291. doi:10.1002/pro.2702
  115. Ph-induced molecular shedding drives the formation of amyloid fibril-derived oligomers. Tipping, Kevin W. , Karamanos, Theodoros K. , Jakhria, Toral , Iadanza, Matthew G. , Goodchild, Sophia C. , Tuma, Roman , Ranson, Neil A. , Hewitt, Eric W. and Radford, Sheena E. (2015) Proceedings of the National Academy of Sciences of the United States of America, 112, 5691-5696. doi:10.1073/pnas.1423174112
  116. Examination of ataxin-3 (atx-3) aggregation by structural mass spectrometry techniques: a rationale for expedited aggregation upon polyglutamine (polyq) expansion. Scarff, Charlotte A. , Almeida, Bruno , Fraga, Joana , Macedo-Ribeiro, S,ra , Radford, Sheena E. and Ashcroft, Alison E. (2015) Molecular & Cellular Proteomics, 14, 1241-1253. doi:10.1074/mcp.M114.044610
  117. Extraction of accurate biomolecular parameters from single-molecule force spectroscopy experiments. Farrance, Oliver E. , Paci, Emanuele , Radford, Sheena E. and Brockwell, David J. (2015) ACS Nano, 9, 1315-1324. doi:10.1021/nn505135d
  118. Force-induced remodelling of proteins and their complexes. Chen, Yun , Radford, Sheena E. and Brockwell, David J. (2015) Current Opinion in Structural Biology, 30, 89-99. doi:10.1016/j.sbi.2015.02.001
  119. Mutational analysis of the ability of resveratrol to inhibit amyloid formation by islet amyloid polypeptide: critical evaluation of the importance of aromatic-inhibitor and histidine-inhibitor interactions. Tu, Ling-Hsien , Young, Lydia M. , Wong, Amy G. , Ashcroft, Alison E. , Radford, Sheena E. and Raleigh, Daniel P. (2015) Biochemistry, 54, 666-676. doi:10.1021/bi501016r
  120. Amphipols outperform dodecylmaltoside micelles in stabilizing membrane protein structure in the gas phase. Calabrese, Antonio N. , Watkinson, Thomas G. , Henderson, Peter J. F. , Radford, Sheena E. and Ashcroft, Alison E. (2015) Analytical Chemistry, 87, 1118-1126. doi:10.1021/ac5037022
  121. Insights into the consequences of co-polymerisation in the early stages of iapp and a beta peptide assembly from mass spectrometry. Young, Lydia M. , Mahood, Rachel A. , Saunders, Janet C. , Tu, Ling-Hsien , Raleigh, Daniel P. , Radford, Sheena E. and Ashcroft, Alison E. (2015) Analyst, 14, 6990-6999. doi:10.1039/c5an00865d
  122. Screening and classifying small-molecule inhibitors of amyloid formation using ion mobility spectrometry-mass spectrometry. Young, Lydia M. , Saunders, Janet C. , Mahood, Rachel A. , Revill, Charlotte H. , Foster, Richard J. , Tu, Ling-Hsien , Raleigh, Daniel P. , Radford, Sheena E. and Ashcroft, Alison E. (2015) Nature Chemistry, 7, 73-81. doi:10.1038/NCHEM.2129
  123. Beta(2)-microglobulin amyloid fibrils are nanoparticles that disrupt lysosomal membrane protein trafficking and inhibit protein degradation by lysosomes. Jakhria, Toral , Hellewell, Andrew L. , Porter, Morwenna Y. , Jackson, Matthew P. , Tipping, Kevin W. , Xue, Wei-Feng , Radford, Sheena E. and Hewitt, Eric W. (2014) Journal of Biological Chemistry, 289, 35781-35794. doi:10.1074/jbc.M114.586222
  124. Mechanistic studies of the biogenesis and folding of outer membrane proteins in vitro and in vivo: what have we learned to date?. McMorran, Lindsay M. , Brockwell, David J. and Radford, Sheena E. (2014) Archives of Biochemistry and Biophysics, 564, 265-280. doi:10.1016/j.abb.2014.02.011
  125. How tric folds tricky proteins. Zhuravleva, Anastasia and Radford, Sheena E. (2014) Cell, 159, 1251-1252. doi:10.1016/j.cell.2014.11.029
  126. Energy landscapes of functional proteins are inherently risky. Gershenson, Anne , Gierasch, Lila M. , Pastore, Annalisa and Radford, Sheena E. (2014) Nature Chemical Biology, 10, 884-891. doi:10.1038/nchembio.1670
  127. Synthesis, characterization and applications of a perdeuterated amphipol. Giusti, Fabrice , Rieger, Jutta , Catoire, Laurent J. , Qian, Shuo , Calabrese, Antonio N. , Watkinson, Thomas G. , Casiraghi, Marina , Radford, Sheena E. , Ashcroft, Alison E. and Popot, Jean-Luc (2014) Journal of Membrane Biology, 247, 909-924. doi:10.1007/s00232-014-9656-x
  128. Distinguishing closely related amyloid precursors using an RNA aptamer. Sarell, Claire J. , Karamanos, Theodoros K. , White, Simon J. , Bunka, David H. J. , Kalverda, Arnout P. , Thompson, Gary S. , Barker, Amy M. , Stockley, Peter G. and Radford, Sheena E. (2014) Journal of Biological Chemistry, 289, 26859-26871. doi:10.1074/jbc.M114.595066
  129. Rare events, yet increasing opportunities: towards combatting amyloid disease. Radford, S. E. (2014) Febs Journal, 281, 62. doi:
  130. Beta(2)-microglobulin amyloid fibril-induced membrane disruption is enhanced by endosomal lipids and acidic ph. Goodchild, Sophia C. , Sheynis, Tania , Thompson, Rebecca , Tipping, Kevin W. , Xue, Wei-Feng , Ranson, Neil A. , Beales, Paul A. , Hewitt, Eric W. and Radford, Sheena E. (2014) Plos One, 9, . doi:10.1371/journal.pone.0104492
  131. Visualization of transient protein-protein interactions that promote or inhibit amyloid assembly. Karamanos, Theodoros K. , Kalverda, Arnout P. , Thompson, Gary S. and Radford, Sheena E. (2014) Molecular Cell, 55, 214-226. doi:10.1016/j.molcel.2014.05.026
  132. Secondary structure in the core of amyloid fibrils formed from human beta(2)m and its truncated variant Delta N6. Su, Yongchao , Sarell, Claire J. , Eddy, Matthew T. , Debelouchina, Galia T. , Andreas, Loren B. , Pashley, Clare L. , Radford, Sheena E. and Griffin, Robert G. (2014) Journal of the American Chemical Society, 136, 6313-6325. doi:10.1021/ja4126092
  133. The role of high-dimensional diffusive search, stabilization, and frustration in protein folding. Rimratchada, Supreecha , McLeish, Tom C. B. , Radford, Sheena E. and Paci, Emanuele (2014) Biophysical Journal, 106, 1729-1740. doi:10.1016/j.bpj.2014.01.051
  134. Analysis of amyloid nanostructures using photo-cross-linking: in situ comparison of three widely used photo-cross-linkers. Preston, George W. , Radford, Sheena E. , Ashcroft, Alison. E. and Wilson, Andrew J. (2014) Acs Chemical Biology, 9, 761-768. doi:10.1021/cb400731s
  135. Investigating protein-protein interaction networks with force spectroscopy. Farrance, Oliver E. , Kaminska, Renata , Housden, Nicholas G. , Derrington, Sasha R. , Kleanthous, Colin , Radford, Sheena E. and Brockwell, David J. (2014) Biophysical Journal, 106, 385A-386A. doi:10.1016/j.bpj.2013.11.2181
  136. Ion mobility spectrometry-mass spectrometry defines the oligomeric intermediates in amylin amyloid formation and the mode of action of inhibitors. Young, Lydia M. , Cao, Ping , Raleigh, Daniel P. , Ashcroft, Alison E. and Radford, Sheena E. (2014) Journal of the American Chemical Society, 136, 660-670. doi:10.1021/ja406831n
  137. Insights into the role of the beta-2 microglobulin d-strand in amyloid propensity revealed by mass spectrometry. Leney, Aneika C. , Pashley, Clare L. , Scarff, Charlotte A. , Radford, Sheena E. and Ashcroft, Alison E. (2014) Molecular Biosystems, 10, 412-420. doi:10.1039/c3mb70420c
  138. An imaging and systems modeling approach to fibril breakage enables prediction of amyloid behavior. Xue, Wei-Feng and Radford, Sheena E. (2013) Biophysical Journal, 105, 2811-2819. doi:10.1016/j.bpj.2013.10.034
  139. Conformational dynamics is more important than helical propensity for the folding of the all alpha-helical protein Im7. Figueiredo, Angelo Miguel , Whittaker, Sara B. -M. , Knowling, Stuart E. , Radford, Sheena E. and Moore, Geoffrey R. (2013) Protein Science, 22, 1722-1738. doi:10.1002/pro.2372
  140. Dissecting the effects of periplasmic chaperones on the in vitro folding of the outer membrane protein pagp. McMorran, Lindsay M. , Bartlett, Alice L. , Huysmans, Gerard H. M. , Radford, Sheena E. and Brockwell, David J. (2013) Journal of Molecular Biology, 425, 3178-3191. doi:10.1016/j.jmb.2013.06.017
  141. Assessing the causes and consequences of co-polymerization in amyloid formation. Sarell, Claire J. , Stockley, Peter G. and Radford, Sheena E. (2013) Prion, 7, 359-368. doi:10.4161/pri.26415
  142. Aggregation modulators interfere with membrane interactions of beta(2)-microglobulin fibrils. Sheynis, Tania , Friediger, Anat , Xue, Wei-Feng , Hellewell, Andrew L. , Tipping, Kevin W. , Hewitt, Eric W. , Radford, Sheena E. and Jelinek, Raz (2013) Biophysical Journal, 105, 745-755. doi:10.1016/j.bpj.2013.06.015
  143. Molecular dynamics simulations of mechanical failure in polymorphic arrangements of amyloid fibrils containing structural defects. Ndlovu, Hlengisizwe , Ashcroft, Alison E. , Radford, Sheena E. and Harris, Sarah A. (2013) Beilstein Journal of Nanotechnology, 4, 429-440. doi:10.3762/bjnano.4.50
  144. A tale of a tail: structural insights into the conformational properties of the polyglutamine protein ataxin-3. Scarff, Charlotte A. , Sicorello, Aless,ro , Tome, Ricardo J. L. , Macedo-Ribeiro, S,ra , Ashcroft, Alison E. and Radford, Sheena E. (2013) international Journal of Mass Spectrometry, 345, 63-70. doi:10.1016/j.ijms.2012.08.032
  145. Advances in ion mobility spectrometry-mass spectrometry reveal key insights into amyloid assembly. Woods, L. A. , Radford, S. E. and Ashcroft, A. E. (2013) Biochimica Et Biophysica Acta-proteins and Proteomics, 1834, 1257-1268. doi:10.1016/j.bbapap.2012.10.002
  146. Expanding the repertoire of amyloid polymorphs by co-polymerization of related protein precursors. Sarell, Claire J. , Woods, Lucy A. , Su, Yongchao , Debelouchina, Galia T. , Ashcroft, Alison E. , Griffin, Robert G. , Stockley, Peter G. and Radford, Sheena E. (2013) Journal of Biological Chemistry, 288, 7327-7337. doi:10.1074/jbc.M112.447524
  147. A force-activated trip switch triggers rapid dissociation of a colicin from its immunity protein. Farrance, Oliver E. , Hann, Eleanore , Kaminska, Renata , Housden, Nicholas G. , Derrington, Sasha R. , Kleanthous, Colin , Radford, Sheena E. and Brockwell, David J. (2013) Plos Biology, 11, . doi:10.1371/journal.pbio.1001489
  148. Direct three-dimensional visualization of membrane disruption by amyloid fibrils. Milanesi, Lilia , Sheynis, Tania , Xue, Wei-Feng , Orlova, Elena V. , Hellewell, Andrew L. , Jelinek, Raz , Hewitt, Eric W. , Radford, Sheena E. and Saibil, Helen R. (2012) Proceedings of the National Academy of Sciences of the United States of America, 109, 20455-20460. doi:10.1073/pnas.1206325109
  149. Amphipathic polymers enable the study of functional membrane proteins in the gas phase. Leney, Aneika C. , McMorran, Lindsay M. , Radford, Sheena E. and Ashcroft, Alison E. (2012) Analytical Chemistry, 84, 9841-9847. doi:10.1021/ac302223s
  150. Molecular insights into aberrant protein self-assembly. Radford, S. E. (2012) Febs Journal, 279, 50-51. doi:
  151. The role of conformational flexibility in beta(2)-microglobulin amyloid fibril formation at neutral ph. Hodkinson, John P. , Radford, Sheena E. and Ashcroft, Alison E. (2012) Rapid Communications in Mass Spectrometry, 26, 1783-1792. doi:10.1002/rcm.6282
  152. Special issue: the molecular and cellular mechanisms of amyloidosis. Radford, Sheena E. and Weissman, Jonathan S. (2012) Journal of Molecular Biology, 421, 139-141. doi:10.1016/j.jmb.2012.05.042
  153. Covalent cross-linking within supramolecular peptide structures. Preston, George W. , Radford, Sheena E. , Ashcroft, Alison E. and Wilson, Andrew J. (2012) Analytical Chemistry, 84, 6790-6797. doi:10.1021/ac301198c
  154. The role of chaperone-subunit usher domain interactions in the mechanism of bacterial pilus biogenesis revealed by ESI-MS. Morrissey, Bethny , Leney, Aneika C. , Rego, Ana Toste , Gilles Phan , Allen, William J. , Verger, Denis , Waksman, Gabriel , Ashcroft, Alison E. and Radford, Sheena E. (2012) Molecular & Cellular Proteomics, 11, . doi:10.1074/mcp.M111.015289
  155. N-terminal acetylation of alpha-synuclein induces increased transient helical propensity and decreased aggregation rates in the intrinsically disordered monomer. Kang, Lijuan , Moriarty, Gina M. , Woods, Lucy A. , Ashcroft, Alison E. , Radford, Sheena E. and Baum, Jean (2012) Protein Science, 21, 911-917. doi:10.1002/pro.2088
  156. Malleability of the folding mechanism of the outer membrane protein pagp: parallel pathways and the effect of membrane elasticity. Huysmans, Gerard H. M. , Radford, Sheena E. , Baldwin, Stephen A. and Brockwell, David J. (2012) Journal of Molecular Biology, 416, 453-464. doi:10.1016/j.jmb.2011.12.039
  157. Conformational properties of the unfolded state of Im7 in nondenaturing conditions. Pashley, Clare L. , Morgan, Gareth J. , Kalverda, Arnout P. , Thompson, Gary S. , Kleanthous, Colin and Radford, Sheena E. (2012) Journal of Molecular Biology, 416, 300-318. doi:10.1016/j.jmb.2011.12.041
  158. Effect of sequence variation on the mechanical response of amyloid fibrils probed by steered molecular dynamics simulation. Ndlovu, Hlengisizwe , Ashcroft, Alison E. , Radford, Sheena E. and Harris, Sarah A. (2012) Biophysical Journal, 102, 587-596. doi:10.1016/j.bpj.2011.12.047
  159. Phosphorylation as a tool to modulate aggregation propensity and to predict fibril architecture. Valette, Nathalie M. , Radford, Sheena E. , Harris, Sarah A. and Warriner, Stuart L. (2012) Chembiochem, 13, 271-281. doi:10.1002/cbic.201100607
  160. Site-specific identification of an a ss fibril-heparin interaction site by using solid-state NMR spectroscopy. Madine, Jillian , P,ya, Maya J. , Hicks, Matthew R. , Rodger, Alison , Yates, Edwin A. , Radford, Sheena E. and Middleton, David A. (2012) Angewandte Chemie-international Edition, 51, 13140-13143. doi:10.1002/anie.201204459
  161. Characterization of the response of primary cells relevant to dialysis-related amyloidosis to beta(2)-microglobulin monomer and fibrils. Porter, Morwenna Y. , Routledge, Katy E. , Radford, Sheena E. and Hewitt, Eric W. (2011) Plos One, 6, . doi:10.1371/journal.pone.0027353
  162. Understanding the complex mechanisms of beta(2)-microglobulin amyloid assembly. Eichner, Timo and Radford, Sheena E. (2011) Febs Journal, 278, 3868-3883. doi:10.1111/j.1742-4658.2011.08186.x
  163. Ligand binding to distinct states diverts aggregation of an amyloid-forming protein. Woods, Lucy A. , Platt, Geoffrey W. , Hellewell, Andrew L. , Hewitt, Eric W. , Homans, Steve W. , Ashcroft, Alison E. and Radford, Sheena E. (2011) Nature Chemical Biology, 7, 730-739. doi:10.1038/NCHEMBIO.635
  164. Structure and dynamics of oligomeric intermediates in beta(2)-microglobulin self-assembly. Smith, David P. , Woods, Lucy A. , Radford, Sheena E. and Ashcroft, Alison E. (2011) Biophysical Journal, 101, 1238-1247. doi:10.1016/j.bpj.2011.07.023
  165. A diversity of assembly mechanisms of a generic amyloid fold. Eichner, Timo and Radford, Sheena E. (2011) Molecular Cell, 43, 8-18. doi:10.1016/j.molcel.2011.05.012
  166. Second order rate constants of donor-strand exchange reveal individual amino acid residues important in determining the subunit specificity of pilus biogenesis. Leney, Aneika C. , Phan, Gilles , Allen, William , Verger, Denis , Waksman, Gabriel , Radford, Sheena E. and Ashcroft, Alison E. (2011) Journal of the American Society for Mass Spectrometry, 22, 1214-1223. doi:10.1007/s13361-011-0146-4
  167. Dissecting key residues in folding and stability of the bacterial immunity protein 7. Knowling, Stuart , Bartlett, Alice I. and Radford, Sheena E. (2011) Protein Engineering Design & Selection, 24, 517-523. doi:10.1093/protein/gzr009
  168. Systematic examination of polymorphism in amyloid fibrils by molecular-dynamics simulation. Berryman, Joshua T. , Radford, Sheena E. and Harris, Sarah A. (2011) Biophysical Journal, 100, 2234-2242. doi:10.1016/j.bpj.2011.02.060
  169. A structural model for apolipoprotein c-ii amyloid fibrils: experimental characterization and molecular dynamics simulations. Teoh, Chai Lean , Pham, Chi L. L. , Todorova, Nevena , Hung, Andrew , Lincoln, Craig N. , Lees, Emma , Lam, Yuen Han , Binger, Katrina J. , Thomson, Neil H. , Radford, Sheena E. , Smith, Trevor A. , Mueller, Shirley A. , Engel, Andreas , Griffin, Michael D. W. , Yarovsky, Irene , Gooley, Paul R. and Howlett, Geoffrey J. (2011) Journal of Molecular Biology, 405, 1246-1266. doi:10.1016/j.jmb.2010.12.006
  170. Protein folding and binding: from biology to physics and back again. Gilson, Michael K. and Radford, Sheena E. (2011) Current Opinion in Structural Biology, 21, 1-3. doi:10.1016/j.sbi.2010.12.003
  171. The oligomeric state and arrangement of the active bacterial translocon. Deville, Karine , Gold, Vicki A. M. , Robson, Alice , Whitehouse, Sarah , Sessions, Richard B. , Baldwin, Stephen A. , Radford, Sheena E. and Collinson, Ian (2011) Journal of Biological Chemistry, 286, 4659-4669. doi:10.1074/jbc.M110.175638
  172. Conformational conversion during amyloid formation at atomic resolution. Eichner, Timo , Kalverda, Arnout P. , Thompson, Gary S. , Homans, Steve W. and Radford, Sheena E. (2011) Molecular Cell, 41, 161-172. doi:10.1016/j.molcel.2010.11.028
  173. Quantifying heterogeneity and conformational dynamics from single molecule fret of diffusing molecules: recurrence analysis of single particles (rasp). Hoffmann, Armin , Nettels, Daniel , Clark, Jennifer , Borgia, Aless,ro , Radford, Sheena E. , Clarke, Jane and Schuler, Benjamin (2011) Physical Chemistry Chemical Physics, 13, 1857-1871. doi:10.1039/c0cp01911a
  174. Perturbing the folding energy landscape of the bacterial immunity protein Im7 by site-specific N-linked glycosylation. Chen, Mark M. , Bartlett, Alice I. , Nerenberg, Paul S. , Friel, Claire T. , Hackenberger, Christian P. R. , Stultz, Collin M. , Radford, Sheena E. and Imperiali, Barbara (2010) Proceedings of the National Academy of Sciences of the United States of America, 107, 22528-22533. doi:10.1073/pnas.1015356107
  175. Intermolecular alignment in beta(2)-microglobulin amyloid fibrils. Debelouchina, Galia T. , Platt, Geoffrey W. , Bayro, Marvin J. , Radford, Sheena E. and Griffin, Robert G. (2010) Journal of the American Chemical Society, 132, 17077-17079. doi:10.1021/ja107987f
  176. Studying peptide aggregation with a series of phosphopeptide variants. Valette, Nathalie M. , Radford, Sheena E. , Harris, Sarah A. and Warriner, Stuart L. (2010) Abstracts of Papers of the American Chemical Society, 240, . doi:
  177. Magic angle spinning NMR analysis of beta(2)-microglobulin amyloid fibrils in two distinct morphologies. Debelouchina, Galia T. , Platt, Geoffrey W. , Bayro, Marvin J. , Radford, Sheena E. and Griffin, Robert G. (2010) Journal of the American Chemical Society, 132, 10414-10423. doi:10.1021/ja102775u
  178. Stacked sets of parallel, in-register beta-strands of beta(2)-microglobulin in amyloid fibrils revealed by site-directed spin labeling and chemical labeling. Ladner, Carol L. , Chen, Min , Smith, David P. , Platt, Geoffrey W. , Radford, Sheena E. and Langen, Ralf (2010) Journal of Biological Chemistry, 285, 17137-17147. doi:10.1074/jbc.M110.117234
  179. Single-molecule studies of the Im7 folding landscape. Pugh, Sara D. , Gell, Christopher , Smith, D. Alastair , Radford, Sheena E. and Brockwell, David J. (2010) Journal of Molecular Biology, 398, 132-145. doi:10.1016/j.jmb.2010.02.048
  180. Elongated oligomers in beta(2)-microglobulin amyloid assembly revealed by ion mobility spectrometry-mass spectrometry. Smith, David P. , Radford, Sheena E. and Ashcroft, Alison E. (2010) Proceedings of the National Academy of Sciences of the United States of America, 107, 6794-6798. doi:10.1073/pnas.0913046107
  181. Desolvation and development of specific hydrophobic core packing during Im7 folding. Bartlett, Alice I. and Radford, Sheena E. (2010) Journal of Molecular Biology, 396, 1329-1345. doi:10.1016/j.jmb.2009.12.048
  182. The transition state for folding of an outer membrane protein. Huysmans, Gerard H. M. , Baldwin, Stephen A. , Brockwell, David J. and Radford, Sheena E. (2010) Proceedings of the National Academy of Sciences of the United States of America, 107, 4099-4104. doi:10.1073/pnas.0911904107
  183. Fibril fragmentation in amyloid assembly and cytotoxicity when size matters. Xue, Wei-Feng , Hellewell, Andrew L. , Hewitt, Eric W. and Radford, Sheena E. (2010) Prion, 4, 20-25. doi:10.4161/pri.4.1.11378
  184. Optimizing protein stability in vivo. Foit, Linda , Morgan, Gareth J. , Kern, Maximilian J. , Steimer, Lenz R. , von Hacht, Annekathrin A. , Titchmarsh, James , Warriner, Stuart L. , Radford, Sheena E. and Bardwell, James C. A. (2009) Molecular Cell, 36, 861-871. doi:10.1016/j.molcel.2009.11.022
  185. Fibril fragmentation enhances amyloid cytotoxicity. Xue, Wei-Feng , Hellewell, Andrew L. , Gosal, Walraj S. , Homans, Steve W. , Hewitt, Eric W. and Radford, Sheena E. (2009) Journal of Biological Chemistry, 284, 34272-34282. doi:10.1074/jbc.M109.049809
  186. Tuning the elastic modulus of hydrated collagen fibrils. Grant, Colin A. , Brockwell, David J. , Radford, Sheena E. and Thomson, Neil H. (2009) Biophysical Journal, 97, 2985-2992. doi:10.1016/j.bpj.2009.09.010
  187. Identification of a mechanical rheostat in the hydrophobic core of protein l. Sadler, David P. , Petrik, Eva , Taniguchi, Yukinori , Pullen, James R. , Kawakami, Masaru , Radford, Sheena E. and Brockwell, David J. (2009) Journal of Molecular Biology, 393, 237-248. doi:10.1016/j.jmb.2009.08.015
  188. Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin e7 immunity protein Im7. Knowling, Stuart E. , Figueiredo, Angelo Miguel , Whittaker, Sara B-M. , Moore, Geoffrey R. and Radford, Sheena E. (2009) Journal of Molecular Biology, 392, 1074-1086. doi:10.1016/j.jmb.2009.07.085
  189. Glimpses of the molecular mechanisms of beta(2)-microglobulin fibril formation in vitro: aggregation on a complex energy landscape. Platt, Geoffrey W. and Radford, Sheena E. (2009) Febs Letters, 583, 2623-2629. doi:10.1016/j.febslet.2009.05.005
  190. Structural insights into the self-assembly mechanism of amyloid fibrils. Radford, Sheena E. (2009) Abstracts of Papers of the American Chemical Society, 238, . doi:
  191. Amyloid fibril length distribution quantified by atomic force microscopy single-particle image analysis. Xue, Wei-Feng , Homans, Steve W. and Radford, Sheena E. (2009) Protein Engineering Design & Selection, 22, 489-496. doi:10.1093/protein/gzp026
  192. Thermodynamic description of polymorphism in q- and n-rich peptide aggregates revealed by atomistic simulation. Berryman, Joshua T. , Radford, Sheena E. and Harris, Sarah A. (2009) Biophysical Journal, 97, 1-11. doi:10.1016/j.bpj.2009.03.062
  193. Competition between intramolecular and intermolecular interactions in an amyloid-forming protein. Routledge, Katy E. , Tartaglia, Gian Gaetano , Platt, Geoffrey W. , Vendrusco, Michele and Radford, Sheena E. (2009) Journal of Molecular Biology, 389, 776-786. doi:10.1016/j.jmb.2009.04.042
  194. An expanding arsenal of experimental methods yields an explosion of insights into protein folding mechanisms. Bartlett, Alice I. and Radford, Sheena E. (2009) Nature Structural & Molecular Biology, 16, 582-588. doi:10.1038/nsmb.1592
  195. Globular tetramers of beta(2)-microglobulin assemble into elaborate amyloid fibrils. White, Helen E. , Hodgkinson, Julie L. , Jahn, Thomas R. , Cohen-Krausz, Sara , Gosal, Walraj S. , Mueller, Shirley , Orlova, Elena V. , Radford, Sheena E. and Saibil, Helen R. (2009) Journal of Molecular Biology, 389, 48-57. doi:10.1016/j.jmb.2009.03.066
  196. A generic mechanism of beta(2)-microglobulin amyloid assembly at neutral pH involving a specific proline switch. Eichner, Timo and Radford, Sheena E. (2009) Journal of Molecular Biology, 386, 1312-1326. doi:10.1016/j.jmb.2009.01.013
  197. The mechanism of folding of Im7 reveals competition between functional and kinetic evolutionary constraints. Friel, Claire T. , Smith, D. Alastair , Vendruscolo, Michele , Gsponer, Joerg and Radford, Sheena E. (2009) Nature Structural & Molecular Biology, 16, 318-324. doi:10.1038/nsmb.1562
  198. Solid-state NMR investigation of b2-microglobulin fibril structure and dynamics. Debelouchina, Galia T. , Bayro, Marvin J. , Platt, Geoffrey W. , Radford, Sheena E. and Griffin, Robert G. (2009) Biophysical Journal, 96, 86A. doi:
  199. HDX-ESI-MS reveals enhanced conformational dynamics of the amyloidogenic protein beta(2)-microglobulin upon release from the mhc-1. Hodkinson, John P. , Jahn, Thomas R. , Radford, Sheena E. and Ashcroft, Alison E. (2009) Journal of the American Society for Mass Spectrometry, 20, 278-286. doi:10.1016/j.jasms.2008.10.005
  200. Probing dynamics within amyloid fibrils using a novel capping method. Platt, Geoffrey W. , Xue, Wei-Feng , Homans, Steve W. and Radford, Sheena E. (2009) Angewandte Chemie-international Edition, 48, 5705-5707. doi:10.1002/anie.200901343
  201. Deciphering drift time measurements from travelling wave ion mobility spectrometry-mass spectrometry studies. Smith, David P. , Knapman, Tom W. , Campuzano, Iain , Malham, Richard W. , Berryman, Joshua T. , Radford, Sheena E. and Ashcroft, Atison E. (2009) European Journal of Mass Spectrometry, 15, 113-130. doi:10.1255/ejms.947
  202. Structural insights into the polymorphism of amyloid-like fibrils formed by region 20-29 of amylin revealed by solid-state NMR and X-ray fiber diffraction. Madine, Jillian , Jack, Edward , Stockley, Peter G. , Radford, Sheena E. , Serpell, Louise C. and Middleton, David A. (2008) Journal of the American Chemical Society, 130, 14990-15001. doi:10.1021/ja802483d
  203. Structural determinants of polymerization reactivity of the P pilus adaptor subunit PapF. Verger, Denis , Rose, Rebecca J. , Paci, Emanuele , Costakes, Greg , Daviter, Tina , Hultgren, Scott , Remaut, Han , Ashcroft, Alison E. , Radford, Sheena E. and Waksman, Gabriel (2008) Structure, 16, 1724-1731. doi:10.1016/j.str.2008.08.012
  204. Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry. Rose, Rebecca J. , Verger, Denis , Daviter, Tina , Remaut, Han , Paci, Emanuele , Waksman, Gabriel , Ashcroft, Alison E. and Radford, Sheena E. (2008) Proceedings of the National Academy of Sciences of the United States of America, 105, 12873-12878. doi:10.1073/pnas.0802177105
  205. Systematic analysis of nucleation-dependent polymerization reveals new insights into the mechanism of amyloid self-assembly. Xue, Wei-Feng , Homans, Steve W. and Radford, Sheena E. (2008) Proceedings of the National Academy of Sciences of the United States of America, 105, 8926-8931. doi:10.1073/pnas.0711664105
  206. A common beta-sheet architecture underlies in vitro and in vivo beta(2)-microglobulin amyloid fibrils. Jahn, Thomas R. , Tennent, Glenys A. and Radford, Sheena E. (2008) Journal of Biological Chemistry, 283, 17279-17286. doi:10.1074/jbc.M710351200
  207. Effects of hydration on the mechanical response of individual collagen fibrils. Grant, Colin A. , Brockwell, David J. , Radford, Sheena E. and Thomson, Neil H. (2008) Applied Physics Letters, 92, . doi:10.1063/1.2937001
  208. Fibril growth kinetics reveal a region of beta(2)-microglobulin important for nucleation and elongation of aggregation. Platt, Geoffrey W. , Routledge, Katy E. , Homans, Steve W. and Radford, Sheena E. (2008) Journal of Molecular Biology, 378, 251-263. doi:10.1016/j.jmb.2008.01.092
  209. Donor-strand exchange in chaperone-assisted pilus assembly revealed in atomic detail by molecular dynamics. Rose, Rebecca J. , Welsh, Thomas S. , Waksman, Gabriel , Ashcroft, Alison E. , Radford, Sheena E. and Paci, Emanuele (2008) Journal of Molecular Biology, 375, 908-919. doi:10.1016/j.jmb.2007.10.077
  210. Folding versus aggregation: polypeptide conformations on competing pathways. Jahn, Thomas R. and Radford, Sheena E. (2008) Archives of Biochemistry and Biophysics, 469, 100-117. doi:10.1016/j.abb.2007.05.015
  211. Trifluoromethyldiazirine: an effective photo-induced cross-linking probe for exploring amyloid formation. Smith, David P. , Anderson, Jon , Plante, Jeffrey , Ashcroft, Alison E. , Radford, Sheena E. , Wilson, Andrew J. and Parker, Martin J. (2008) Chemical Communications, , 5728-5730. doi:10.1039/b813504e
  212. InteRNAl friction of single polypeptide chains at high stretch. Khatri, Bhavin S. , Byrne, Katherine , Kawakami, Masaru , Brockwell, David J. , Smith, D. Alastair , Radford, Sheena E. and McLeish, Tom C. B. (2008) Faraday Discussions, 139, 35-51. doi:10.1039/b716418c
  213. Monitoring copopulated conformational states during protein folding events using electrospray ionization-ion mobility spectrometry-mass spectrometry. Smith, David P. , Giles, Kevin , Bateman, Robert H. , Radford, Sheena E. and Ashcroft, Alison E. (2007) Journal of the American Society for Mass Spectrometry, 18, 2180-2190. doi:10.1016/j.jasms.2007.09.017
  214. Production and characterization of RNA aptamers specific for amyloid fibril epitopes. Bunka, David H. J. , Mantle, Benjamin J. , Morten, Isobel J. , Tennent, Glenys A. , Radford, Sheena E. and Stockley, Peter G. (2007) Journal of Biological Chemistry, 282, 34500-34509. doi:10.1074/jbc.M703679200
  215. The N-terminal helix is a post-assembly clamp in the bacterial outer membrane protein pagp. Huysmans, Gerard H. M. , Radford, Sheena E. , Brockwell, David J. and Baldwin, Stephen A. (2007) Journal of Molecular Biology, 373, 529-540. doi:10.1016/j.jmb.2007.07.072
  216. Investigation into the role of macrophages in the formation and degradation of beta(2)-microglobulin amyloid fibrils. Morten, Isobel J. , Gosal, Walraj S. , Radford, Sheena E. and Hewitt, Eric W. (2007) Journal of Biological Chemistry, 282, 29691-29700. doi:10.1074/jbc.M705004200
  217. Avoidance of epimerization in the synthesis of peptide thioesters using fmoc protection. Nagalingam, Anil C. , Radford, Sheena E. and Warriner, Stuart L. (2007) Synlett, , 2517-2520. doi:10.1055/s-2007-986631
  218. The effect of increasing the stability of non-native interactions on the folding landscape of the bacterial immunity protein Im9. Morton, Victoria L. , Friel, Claire T. , Allen, Lucy R. , Paci, Emanuele and Radford, Sheena E. (2007) Journal of Molecular Biology, 371, 554-568. doi:10.1016/j.jmb.2007.05.010
  219. Specific glycosaminoglycans promote unseeded amyloid formation from beta(2)-microglobulin under physiological conditions. Borysik, A. J. , Morten, I. J. , Radford, S. E. and Hewitt, E. W. (2007) Kidney international, 72, 174-181. doi:10.1038/sj.ki.5002270
  220. Nucleation of protein fibrillation by nanoparticles. Linse, Sara , Cabaleiro-Lago, Celia , Xue, Wei-Feng , Lynch, Iseult , Lindman, Stina , Thulin, Eva , Radford, Sheena E. and Dawson, Kenneth A. (2007) Proceedings of the National Academy of Sciences of the United States of America, 104, 8691-8696. doi:10.1073/pnas.0701250104
  221. The effect of protein complexation on the mechanical stability of Im9. Hann, Eleanore , Kirkpatrick, Nadine , Kleanthous, Colin , Smith, D. Alastair , Radford, Sheena E. and Brockwell, David J. (2007) Biophysical Journal, 92, L79-L81. doi:10.1529/biophysj.106.102475
  222. Nmr analysis of the conformational properties of the trapped on-pathway folding intermediate of the bacterial immunity protein Im7. Whittaker, Sara B. -M. , Spence, Graham R. , Grossmann, J. Gunter , Radford, Sheena E. and Moore, Geoffrey R. (2007) Journal of Molecular Biology, 366, 1001-1015. doi:10.1016/j.jmb.2006.11.012
  223. Intermediates: ubiquitous species on folding energy landscapes?. Brockwell, David J. and Radford, Sheena E. (2007) Current Opinion in Structural Biology, 17, 30-37. doi:10.1016/j.sbi.2007.01.003
  224. Progressive glycosylation of albumin and its effect on the binding of homocysteine may be a key step in the pathogenesis of vascular damage in diabetes mellitus. Mathai, M. , Radford, S. E. , Holland, P. (2007) Medical Hypotheses, 69, 166-172. doi:10.1016/j.mehy.2006.10.050
  225. Characterisation of the conformational properties of urea-unfolded Im7: implications for the early stages of protein folding. Le Duff, Cecile S. , Whittaker, Sara B. -M. , Radford, Sheena E. and Moore, Geoffrey R. (2006) Journal of Molecular Biology, 364, 824-835. doi:10.1016/j.jmb.2006.09.037
  226. Direct observation of oligomeric species formed in the early stages of amyloid fibril formation using electrospray ionisation mass spectrometry. Smith, Andrew M. , Jahn, Thomas R. , Ashcroft, Alison E. and Radford, Sheena E. (2006) Journal of Molecular Biology, 364, 9-19. doi:10.1016/j.jmb.2006.08.081
  227. Biol 7 - life on a knife edge: tipping the balance between folding and aggregation. Radford, Sheena E. (2006) Abstracts of Papers of the American Chemical Society, 232, 897. doi:
  228. Urea-induced unfolding of the immunity protein Im9 monitored by spFRET. Tezuka-Kawakami, Tomoko , Gell, Chris , Brockwell, David J. , Radford, Sheena E. and Smith, D. Alastair (2006) Biophysical Journal, 91, L42-L44. doi:10.1529/biophysj.106.088344
  229. Viscoelastic study of the mechanical unfolding of a protein by afm. Kawakami, Masaru , Byrne, Katherine , Brockwell, David J. , Radford, Sheena E. and Smith, D. Alastair (2006) Biophysical Journal, 91, L16-L18. doi:10.1529/biophysj.106.085019
  230. The organization of aromatic side groups in an amyloid fibril probed by solid-state 2H and 19F NMR spectroscopy. Jack, E , Newsome, M , Stockley, PG , Radford, SE and Middleton, DA (2006) Journal of the American Chemical Society, 128, 8098-8099. doi:10.1021/ja0581898
  231. Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism. Remaut, Han , Rose, Rebecca J. , Hannan, Thomas J. , Hultgren, Scott J. , Radford, Sheena E. , Ashcroft, Alison E. and Waksman, Gabriel (2006) Molecular Cell, 22, 831-842. doi:10.1016/j.molcel.2006.05.033
  232. GroEL: more than just a folding cage. Radford, Sheena E. (2006) Cell, 125, 831-833. doi:10.1016/j.cell.2006.05.021
  233. Detailed evaluation of the performance of microfluidic t mixers using fluorescence and ultraviolet resonance raman spectroscopy. Masca, Sergiu I. , Rodriguez-Mendieta, Inigo R. , Friel, Claire T. , Radford, Sheena E. and Smith, D. Alastair (2006) Review of Scientific instruments, 77, . doi:10.1063/1.2198800
  234. Amyloid formation under physiological conditions proceeds via a native-like folding intermediate. Jahn, TR , Parker, MJ , Homans, SW and Radford, SE (2006) Nature Structural & Molecular Biology, 13, 195-201. doi:10.1038/nsmb1058
  235. Sulfate-induced effects in the on-pathway intermediate of the bacterial immunity protein Im7. Cobos, ES and Radford, SE (2006) Biochemistry, 45, 2274-2282. doi:10.1021/bi0521238
  236. A systematic study of the effect of physiological factors on beta 2-microglobulin amyloid formation at neutral ph. Myers, SL , Jones, S , Jahn, TR , Morten, IJ , Tennent, GA , Hewitt, EW and Radford, SE (2006) Biochemistry, 45, 2311-2321. doi:10.1021/bi052434i
  237. Determination of an ensemble of structures representing the intermediate state of the bacterial immunity protein Im7. Gsponer, J , Hopearuoho, H , Whittaker, SBM , Spence, GR , Moore, GR , Paci, E , Radford, SE and Vendruscolo, M (2006) Proceedings of the National Academy of Sciences of the United States of America, 103, 99-104. doi:10.1073/pnas.0508667102
  238. Mechanical resistance of proteins explained using simple molecular models. West, DK , Brockwell, DJ , Olmsted, PD , Radford, SE and Paci, E (2006) Biophysical Journal, 90, 287-297. doi:10.1529/biophysj.105.071035
  239. Amyloid under the atomic force microscope. Gosal, WS , Myers, SL , Radford, SE and Thomson, NH (2006) Protein and Peptide Letters, 13, 261-270. doi:10.2174/092986606775338498
  240. Investigating the structural properties of amyloid-like fibrils formed in vitro from beta 2-microglobulin using limited proteolysis and electrospray ionisation mass spectrometry. Myers, Sarah L. , Thomson, Neil H. , Radford, Sheena E. and Ashcroft, Alison E. (2006) Rapid Communications in Mass Spectrometry, 20, 1628-1636. doi:10.1002/rcm.2482
  241. The yin and yang of protein folding. Jahn, TR and Radford, SE (2005) Febs Journal, 272, 5962-5970. doi:10.1111/j.1742-4658.2005.05021.x
  242. Investigation into the role of lysosomes in dialysis related amyloidosis. Morten, IJ , Radford, SE and Hewitt, EW (2005) Immunology, 116, 63. doi:
  243. Towards an understanding of the structural molecular mechanism of beta(2)-microglobulin amyloid formation in vitro. Radford, SE , Gosal, WS and Platt, GW (2005) Biochimica Et Biophysica Acta-proteins and Proteomics, 1753, 51-63. doi:10.1016/j.bbapap.2005.07.006
  244. Semisynthesis of a glycosylated lm7 analogue for protein folding studies. Hackenberger, CPR , Friel, CT , Radford, SE and Imperiali, B (2005) Journal of the American Chemical Society, 127, 12882-12889. doi:10.1021/ja051855k
  245. Competing pathways determine fibril morphology in the self-assembly of beta(2)-microglobulin into amyloid. Gosal, WS , Morten, IJ , Hewitt, EW , Smith, DA , Thomson, NH and Radford, SE (2005) Journal of Molecular Biology, 351, 850-864. doi:10.1016/j.jmb.2005.06.040
  246. Mechanically unfolding the small, topologically simple protein l. Brockwell, DJ , Beddard, GS , Paci, E , West, DK , Olmsted, PD , Smith, DA and Radford, SE (2005) Biophysical Journal, 89, 506-519. doi:10.1529/biophysj.105.061465
  247. Viscoelastic measurements of single molecules on a millisecond time scale by magnetically driven oscillation of an atomic force microscope cantilever. Kawakami, M , Byrne, K , Khatri, BS , Mcleish, TCB , Radford, SE and Smith, DA (2005) Langmuir, 21, 4765-4772. doi:10.1021/la0469699
  248. Semi-synthesis of the immunity protein Im7 to study the effect of glycosylation on protein folding.. Hackenherger, CPR , Friel, CT , Radford, SE and Imperiali, B (2005) Abstracts of Papers of the American Chemical Society, 229, U394. doi:
  249. Ultraviolet resonance raman studies reveal the environment of tryptophan and tyrosine residues in the native and partially folded states of the e colicin-binding immunity protein Im7. Rodriguez-Mendieta, IR , Spence, GR , Gell, C , Radford, SE and Smith, DA (2005) Biochemistry, 44, 3306-3315. doi:10.1021/bi047746k
  250. Protein folding: defining a ``standard'' set of experimental conditions and a preliminary kinetic data set of two-state proteins. Maxwell, KL , Wildes, D , Zarrine-Afsar, A , de los Rios, MA , Brown, AG , Friel, CT , Hedberg, L , Horng, JC , Bona, D , Miller, EJ , Vallee-Belisle, A , Main, ERG , Bemporad, F , Qiu, LL , Teilum, K , Vu, ND , Edwards, AM , Ruczinski, I , Poulsen, FM , Kragelund, BB , Michnick, SW , Chiti, F , Bai, YW , Hagen, SJ , Serrano, L , Oliveberg, M , Raleigh, DP , Wittung-Stafshede, P , Radford, SE , Jackson, SE , Sosnick, TR , Marqusee, S , Davidson, AR and Plaxco, KW (2005) Protein Science, 14, 602-616. doi:10.1110/ps.041205405
  251. Dynamics in the unfolded state of beta(2)-microglobulin studied by NMR. Plattt, GW , McParl,, VJ , Kalverda, AP , Homans, SW and Radford, SE (2005) Journal of Molecular Biology, 346, 279-294. doi:10.1016/j.jmb.2004.11.035
  252. Helix stability and hydrophobicity in the folding mechanism of the bacterial immunity protein Im9. Cranz-Mileva, S , Friel, CT and Radford, SE (2005) Protein Engineering Design & Selection, 18, 41-50. doi:10.1093/protein/gzi002
  253. Viscoelastic properties of single polysaccharide molecules determined by analysis of thermally driven oscillations of an atomic force microscope cantilever. Kawakami, M , Byrne, K , Khatri, B , Mcleish, TCB , Radford, SE and Smith, DA (2004) Langmuir, 20, 9299-9303. doi:10.1021/la0486178
  254. Switching two-state to three-state kinetics in the helical protein Im9 via the optimisation of stabilising non-native interactions by design. Friel, CT , Beddard, GS and Radford, SE (2004) Journal of Molecular Biology, 342, 261-273. doi:10.1016/j.jmb.2004.06.076
  255. Trapping the on-pathway folding intermediate of Im7 at equilibrium. Spence, GR , Capaldi, AP and Radford, SE (2004) Journal of Molecular Biology, 341, 215-226. doi:10.1016/j.jmb.2004.05.049
  256. Co-populated conformational ensembles of beta(2)-microglobulin uncovered quantitatively by electrospray ionization mass spectrometry. Borysik, AJH , Radford, SE and Ashcroft, AE (2004) Journal of Biological Chemistry, 279, 27069-27077. doi:10.1074/jbc.M401472200
  257. Microsecond folding dynamics of the F13W G29A mutant of the b domain of staphylococcal protein a by laser-induced temperature jump. Dimitriadis, G , Drysdale, A , Myers, JK , Arora, P , Radford, SE , Oas, TG and Smith, DA (2004) Proceedings of the National Academy of Sciences of the United States of America, 101, 3809-3814. doi:10.1073/pnas.0306433101
  258. Equilibrium hydrogen exchange reveals extensive hydrogen bonded secondary structure in the on-pathway intermediate of Im7. Gorski, SA , Le Duff, CS , Capaldi, AP , Kalverda, AP , Beddard, GS , Moore, GR and Radford, SE (2004) Journal of Molecular Biology, 337, 183-193. doi:10.1016/j.jmb.2004.01.004
  259. Comparison of the transition state ensembles for folding of Im7 and Im9 determined using all-atom molecular dynamics simulations with phi value restraints. Paci, E , Friel, CT , Lindorff-Larsen, K , Radford, SE , Karplus, M and Vendruscolo, M (2004) Proteins-structure Function and Bioinformatics, 54, 513-525. doi:10.1002/prot.10595
  260. Separation of beta(2)-microglobulin conformers by high-field asymmetric waveform ion mobility spectrometry (faims) coupled to electrospray ionisation mass spectrometry. Borysik, AJH , Read, P , Little, DR , Bateman, RH , Radford, SE and Ashcroft, AE (2004) Rapid Communications in Mass Spectrometry, 18, 2229-2234. doi:10.1002/rcm.1613
  261. Pulling geometry defines the mechanical resistance of a beta-sheet protein (vol 10, pg 731, 2003). Brockwell, DJ , Paci, E , Zinober, RC , Beddard, GS , Olmsted, PD , Smith, DA , Perham, RN and Radford, SE (2003) Nature Structural Biology, 10, 872. doi:10.1038/nsb1003-872b
  262. Beta edge strands in protein structure prediction and aggregation. Siepen, JA , Radford, SE and Westhead, DR (2003) Protein Science, 12, 2348-2359. doi:10.1110/ps.03234503
  263. Pulling geometry defines the mechanical resistance of a beta-sheet protein. Brockwell, DJ , Paci, E , Zinober, RC , Beddard, GS , Olmsted, PD , Smith, DA , Perham, RN and Radford, SE (2003) Nature Structural Biology, 10, 731-737. doi:10.1038/nsb968
  264. Ph as a trigger of peptide beta-sheet self-assembly and reversible switching between nematic and isotropic phases. Aggeli, A , Bell, M , Carrick, LM , Fishwick, CWG , Harding, R , Mawer, PJ , Radford, SE , Strong, AE and Boden, N (2003) Journal of the American Chemical Society, 125, 9619-9628. doi:10.1021/ja021047i
  265. Role of the n and c-terminal strands of beta 2-microglobulin in amyloid formation at neutral ph. Jones, S , Smith, DP and Radford, SE (2003) Journal of Molecular Biology, 330, 935-941. doi:10.1016/S0022-2836(03)00688-0
  266. A systematic investigation into the effect of protein destabilisation on beta 2-microglobulin amyloid formation. Smith, DP , Jones, S , Serpell, LC , Sunde, M and Radford, SE (2003) Journal of Molecular Biology, 330, 943-954. doi:10.1016/S0022-2836(03)00687-9
  267. Hierarchical assembly of beta(2)-microglobulin amyloid in vitro revealed by atomic force microscopy. Kad, NM , Myers, SL , Smith, DP , Smith, DA , Radford, SE and Thomson, NH (2003) Journal of Molecular Biology, 330, 785-797. doi:10.1016/S0022-2836(03)00583-7
  268. Unfolding dynamics of proteins under applied force. Smith, DA , Brockwell, DJ , Zinober, RC , Blake, AW , Beddard, GS , Olmsted, PD and Radford, SE (2003) Philosophical Transactions of the Royal Society A-mathematical Physical and Engineering Sciences, 361, 713-728. doi:10.1098/rsta.2002.1160
  269. Force mode atomic force microscopy as a tool for protein folding studies. Best, RB , Brockwell, DJ , Toca-Herrera, JL , Blake, AW , Smith, DA , Radford, SE and Clarke, J (2003) Analytica Chimica Acta, 479, 87-105. doi:10.1016/S0003-2670(02)01572-6
  270. Co-translocational misfolding in the ER of living cells. Radford, SE (2003) Nature Structural Biology, 10, 153-154. doi:10.1038/nsb0303-153
  271. Structural analysis of the rate-limiting transition states in the folding of lm7 and lm9: similarities and differences in the folding of homologous proteins. Friel, CT , Capaldi, AP and Radford, SE (2003) Journal of Molecular Biology, 326, 293-305. doi:10.1016/S0022-2836(02)01249-4
  272. Amyloid-forming peptides from beta(2)-microglobulin - insights into the mechanism of fibril formation in vitro. Jones, S , Manning, J , Kad, NM and Radford, SE (2003) Journal of Molecular Biology, 325, 249-257. doi:10.1016/S0022-2836(02)01227-5
  273. Mechanically unfolding proteins: the effect of unfolding history and the supramolecular scaffold. Zinober, RC , Brockwell, DJ , Beddard, GS , Blake, AW , Olmsted, PD , Radford, SE and Smith, DA (2002) Protein Science, 11, 2759-2765. doi:10.1110/ps.0224602
  274. Structural plasticity and noncovalent substrate binding in the GroEL apical domain - a study using electrospray ionization mass spectrometry and fluorescence binding studies. Ashcroft, AE , Brinker, A , Coyle, JE , Weber, F , Kaiser, M , Moroder, L , Parsons, MR , Jager, J , Hartl, UF , Hayer-Hartl, M and Radford, SE (2002) Journal of Biological Chemistry, 277, 33115-33126. doi:10.1074/jbc.M203398200
  275. Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties. Trinh, CH , Smith, DP , Kalverda, AP , Phillips, SEV and Radford, SE (2002) Proceedings of the National Academy of Sciences of the United States of America, 99, 9771-9776. doi:10.1073/pnas.152337399
  276. The effect of core destabilization on the mechanical resistance of i27. Brockwell, DJ , Beddard, GS , Clarkson, J , Zinober, RC , Blake, AW , Trinick, J , Olmsted, PD , Smith, DA and Radford, SE (2002) Biophysical Journal, 83, 458-472. doi:10.1016/S0006-3495(02)75182-5
  277. The yeast prion ure2p retains its native alpha-helical conformation upon assembly into protein fibrils in vitro. Bousset, L , Thomson, NH , Radford, SE and Melki, R (2002) Embo Journal, 21, 2903-2911. doi:10.1093/emboj/cdf303
  278. Structural properties of an amyloid precursor of beta(2)-microglobulin. McParl,, VJ , Kalverda, AP , Homans, SW and Radford, SE (2002) Nature Structural Biology, 9, 326-331. doi:10.1038/nsb791
  279. Im7 folding mechanism: misfolding on a path to the native state. Capaldi, AP , Kleanthous, C and Radford, SE (2002) Nature Structural Biology, 9, 209-216. doi:10.1038/nsb757
  280. Beta(2)-microglobulin and its deamidated variant, N17D form amyloid fibrils with a range of morphologies in vitro. Kad, NM , Thomson, NH , Smith, DP , Smith, DA and Radford, SE (2001) Journal of Molecular Biology, 313, 559-571. doi:10.1006/jmbi.2001.5071
  281. Acidic conditions stabilise intermediates populated during the folding of Im7 and Im9. Gorski, SA , Capaldi, AP , Kleanthous, C and Radford, SE (2001) Journal of Molecular Biology, 312, 849-863. doi:10.1006/jmbi.2001.5001
  282. Role of the single disulphide bond of beta(2)-microglobulin in amyloidosis in vitro. Smith, DP and Radford, SE (2001) Protein Science, 10, 1775-1784. doi:10.1110/ps.4901
  283. A partially folded intermediate species of the beta-sheet protein apo-pseudoazurin is trapped during proline-limited folding. Reader, JS , Van Nul,, NAJ , Thompson, GS , Ferguson, SJ , Dobson, CM and Radford, SE (2001) Protein Science, 10, 1216-1224. doi:10.1110/ps.52801
  284. Using chimeric immunity proteins to explore the energy landscape for alpha-helical protein folding. Ferguson, N , Li, W , Capaldi, AP , Kleanthous, C and Radford, SE (2001) Journal of Molecular Biology, 307, 393-405. doi:10.1006/jmbi.2000.4492
  285. Ultrarapid mixing experiments reveal that Im7 folds via an on-pathway intermediate. Capaldi, AP , Shastry, MCR , Kleanthous, C , Roder, H and Radford, SE (2001) Nature Structural Biology, 8, 68-72. doi:
  286. Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times. Gell, C , Brockwell, DJ , Beddard, GS , Radford, SE , Kalverda, AP and Smith, DA (2001) Single Molecules, 2, 177-181. doi:10.1002/1438-5171(200110)2:3<177::AID-SIMO177>3.0.CO;2-4
  287. Protein folding: progress made and promises ahead. Radford, SE (2000) Trends in Biochemical Sciences, 25, 611-618. doi:10.1016/S0968-0004(00)01707-2
  288. Protein folding: pulling back the frontiers. Smith, DA and Radford, SE (2000) Current Biology, 10, R662-R664. doi:10.1016/S0960-9822(00)00681-3
  289. Partially unfolded states of beta(2)-microglobulin and amyloid formation in vitro. McParl,, VJ , Kad, NM , Kalverda, AP , Brown, A , Kirwin-Jones, P , Hunter, MG , Sunde, M and Radford, SE (2000) Biochemistry, 39, 8735-8746. doi:10.1021/bi000276j
  290. Partially unfolded species populated during equilibrium denaturation of the beta-sheet protein Y74W apo-pseudoazurin. Jones, S , Reader, JS , Healy, M , Capaldi, AP , Ashcroft, AE , Kalverda, AP , Smith, DA and Radford, SE (2000) Biochemistry, 39, 5672-5682. doi:10.1021/bi9923959
  291. The structure and dynamics in solution of Cu(I) pseudoazurin from paracoccus pantotrophus. Thompson, GS , Leung, YC , Ferguson, SJ , Radford, SE and Redfield, C (2000) Protein Science, 9, 846-858. doi:10.1110/ps.9.5.846
  292. Ribosome-mediated folding of partially unfolded ricin a-chain. Argent, RH , Parrott, AM , Day, PJ , Roberts, LM , Stockley, PG , Lord, JM and Radford, SE (2000) Journal of Biological Chemistry, 275, 9263-9269. doi:10.1074/jbc.275.13.9263
  293. Thermal unfolding of an intermediate is associated with non-arrhenius kinetics in the folding of hen lysozyme. Matagne, A , Jamin, M , Chung, EW , Robinson, CV , Radford, SE and Dobson, CM (2000) Journal of Molecular Biology, 297, 193-210. doi:10.1006/jmbi.2000.3540
  294. Protein folding mechanisms: new methods and emerging ideas. Brockwell, DJ , Smith, DA and Radford, SE (2000) Current Opinion in Structural Biology, 10, 16-25. doi:10.1016/S0959-440X(99)00043-3
  295. GroEL accelerates the refolding of hen lysozyme without changing its folding mechanism. Coyle, JE , Texter, FL , Ashcroft, AE , Masselos, D , Robinson, CV and Radford, SE (1999) Nature Structural Biology, 6, 683-690. doi:
  296. From computer simulations to human disease: emerging themes in protein folding. Radford, SE and Dobson, CM (1999) Cell, 97, 291-298. doi:10.1016/S0092-8674(00)80739-4
  297. Rapid folding with and without populated intermediates in the homologous four-helix proteins Im7 and Im9. Ferguson, N , Capaldi, AP , James, R , Kleanthous, C and Radford, SE (1999) Journal of Molecular Biology, 286, 1597-1608. doi:10.1006/jmbi.1998.2548
  298. The greek key protein apo-pseudoazurin folds through an obligate on-pathway intermediate. Capaldi, AP , Ferguson, SJ and Radford, SE (1999) Journal of Molecular Biology, 286, 1621-1632. doi:10.1006/jmbi.1998.2588
  299. Structural and mechanistic basis of immunity toward endonuclease colicins. Kleanthous, C , Kuhlmann, UC , Pommer, AJ , Ferguson, N , Radford, SE , Moore, GR , James, R and Hemmings, AM (1999) Nature Structural Biology, 6, 243-252. doi:10.1038/6683
  300. Insights into the molecular mechanisms of protein folding and misfolding. Radford, SE (1999) , , 352-354. doi:
  301. A near-native state on the slow refolding pathway of hen lysozyme. Kulkarni, SK , Ashcroft, AE , Carey, M , Masselos, D , Robinson, CV and Radford, SE (1999) Protein Science, 8, 35-44. doi:
  302. The origin of the alpha-domain intermediate in the folding of hen lysozyme. Matagne, A , Chung, EW , Ball, LJ , Radford, SE , Robinson, CV and Dobson, CM (1998) Journal of Molecular Biology, 277, 997-1005. doi:10.1006/jmbi.1998.1657
  303. Kinetic studies of beta-sheet protein folding. Capaldi, AP and Radford, SE (1998) Current Opinion in Structural Biology, 8, 86-92. doi:10.1016/S0959-440X(98)80014-6
  304. Probing conformations of GroEL-bound substrate proteins by mass spectrometry. Robinson, CV , Gross, M and Radford, SE (1998) , 290, 296-313. doi:10.1016/S0076-6879(98)90026-X
  305. Abolition of the fast track of lysozyme folding at neutral ph. Texter, FL , Coyle, JE , Kulkarni, SK , Robinson, CV and Radford, SE (1997) FASEB Journal, 11, A907. doi:
  306. Engineering of peptide beta-sheet nanotapes. Aggeli, A , Bell, M , Boden, N , Keen, JN , McLeish, TCB , Nyrkova, I , Radford, SE and Semenov, A (1997) Journal of Materials Chemistry, 7, 1135-1145. doi:10.1039/a701088e
  307. Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR. Chung, EW , Nettleton, EJ , Morgan, CJ , Gross, M , Miranker, A , Radford, SE , Dobson, CM and Robinson, CV (1997) Protein Science, 6, 1316-1324. doi:10.1002/pro.5560060620
  308. Fast and slow tracks in lysozyme folding: insight into the role of domains in the folding process. Matagne, A , Radford, SE and Dobson, CM (1997) Journal of Molecular Biology, 267, 1068-1074. doi:10.1006/jmbi.1997.0963
  309. Self-assembly of peptides into polymeric beta-sheet tapes.. Agglei, A , Boden, N , McLeish, TCB and Radford, SE (1997) Abstracts of Papers of the American Chemical Society, 213, 368-COLL. doi:
  310. Responsive gels formed by the spontaneous self-assembly of peptides into polymeric beta-sheet tapes. Aggeli, A , Bell, M , Boden, N , Keen, JN , Knowles, PF , McLeish, TCB , Pitkeathly, M and Radford, SE (1997) Nature, 386, 259-262. doi:10.1038/386259a0
  311. Instability, unfolding and aggregation of human lysozyme variants underlying amyloid fibrillogenesis. Booth, DR , Sunde, M , Bellotti, V , Robinson, CV , Hutchinson, WL , Fraser, PE , Hawkins, PN , Dobson, CM , Radford, SE , Blake, CCF and Pepys, MB (1997) Nature, 385, 787-793. doi:10.1038/385787a0
  312. The pseudoazurin gene from thiosphaera pantotropha: analysis of upstream putative regulatory sequences and overexpression in Eschirichia coli. Leung, YC , Chan, C , Reader, JS , Willis, AC , vanSpanning, RJM , Ferguson, SJ and Radford, SE (1997) Biochemical Journal, 321, 699-705. doi:10.1042/bj3210699
  313. Acceleration of the folding of hen lysozyme by trifluoroethanol. Lu, H , Buck, M , Radford, SE and Dobson, CM (1997) Journal of Molecular Biology, 265, 112-117. doi:10.1006/jmbi.1996.0715
  314. Structural and mechanistic consequences of polypeptide binding by GroEL. Coyle, JE , Jaeger, J , Gross, M , Robinson, CV and Radford, SE (1997) Folding & Design, 2, R93-R104. doi:10.1016/S1359-0278(97)00046-1
  315. Significant hydrogen exchange protection in GroEL-bound DHFR is maintained during iterative rounds of substrate cycling. Gross, M , Robinson, CV , Mayhew, M , Hartl, FU and Radford, SE (1996) Protein Science, 5, 2506-2513. doi:10.1002/pro.5560051213
  316. Hen egg white lysozyme: a temperature dependence study of the folding process. Matagne, A , Radford, SE and Dobson, CM (1996) Archives of Physiology and Biochemistry, 104, B45. doi:
  317. Investigation of protein folding by mass spectrometry. Miranker, A , Robinson, CV , Radford, SE and Dobson, CM (1996) FASEB Journal, 10, 93-101. doi:
  318. Native-like secondary structure in a peptide from the alpha-domain of hen lysozyme. Yang, JJ , v,enBerg, B , Pitkeathly, M , Smith, LJ , Bolin, KA , Keiderling, TA , Redfield, C , Dobson, CM and Radford, SE (1996) Folding & Design, 1, 473-484. doi:10.1016/S1359-0278(96)00063-6
  319. Molecular mechanisms of fibrillogenesis and the protective role of amyloid p component: two possible avenues for therapy. Pepys, MB , Tennent, GA , Booth, DR , Bellotti, V , Lovat, LB , Tan, SY , Persey, MR , Hutchinson, WL , Booth, SE , Madhoo, S , Soutar, AK , Hawkins, PN , VanZylSmit, R , Campistol, JM , Fraser, PE , Radford, SE , Robinson, CV , Sunde, M , Serpell, LC , Blake, CCF , Maeda , Goldgaber , Kisilevsky , Buxbaum, JN , Westermark , Sipe , Kirschner , Masters , Caughey , Wood and Kelly (1996) , 199, 73-89. doi:
  320. Pseudospecific docking surfaces on electron-transfer proteins as illustrated by pseudoazurin, cytochrome c(550) and cytochrome cd(1) nitrite reductase. WILLIAMS, PA , FULOP, V , Leung, YC , Chan, C , Moir, JWB , Howlett, G , Ferguson, SJ , Radford, SE and HAJDU, J (1995) Nature Structural Biology, 2, 975-982. doi:10.1038/nsb1195-975
  321. Conformational properties of 4 peptides spanning the sequence of hen lysozyme. Yang, JJ , Buck, M , Pitkeathly, M , Kotik, M , Haynie, DT , Dobson, CM and Radford, SE (1995) Journal of Molecular Biology, 252, 483-491. doi:10.1006/jmbi.1995.0513
  322. Weighing the evidence for structure - electrospray-ionization mass-spectrometry of proteins. Robinson, CV and Radford, SE (1995) Structure, 3, 861-865. doi:10.1016/S0969-2126(01)00221-0
  323. Cooperative elements in protein-folding monitored by electrospray-ionization mass-spectrometry. Hooke, SD , Eyles, SJ , Miranker, A , Radford, SE , Robinson, CV and Dobson, CM (1995) Journal of the American Chemical Society, 117, 7548-7549. doi:10.1021/ja00133a030
  324. The complete amino-acid-sequence confirms the presence of pseudoazurin in thiosphaera-pantotropha. Chan, C , Willis, AC , Robinson, CV , Aplin, RT , Radford, SE and Ferguson, SJ (1995) Biochemical Journal, 308, 585-590. doi:10.1042/bj3080585
  325. Insights into protein-folding using physical techniques - studies of lysozyme and alpha-lactalbumin. Radford, SE and Dobson, CM (1995) Philosophical Transactions of the Royal Society of London Series B-biological Sciences, 348, 17-25. doi:10.1098/rstb.1995.0041
  326. Folding of a partially folded state of hen lysozyme in trifluoroethanol. Hui, Lu , Buck, M , Radford, SE and Dobson, CM (1995) FASEB Journal, 9, A1242. doi:
  327. Folding of hen lysozyme. Lu, H , Radford, SE and Dobson, CM (1995) FASEB Journal, 9, A1469. doi:
  328. Lysozyme - a model protein for folding studies. Radford, SE (1995) FASEB Journal, 9, A1257. doi:
  329. Physicochemical characterization of the cytoplasmic domain of the epidermal growth-factor receptor and evidence for conformational-changes associated with its activation by ammonium-sulfate. Gregoriou, M , Jones, PF , Timms, JF , Yang, JJ , Radford, SE and Rees, AR (1995) Biochemical Journal, 306, 667-678. doi:10.1042/bj3060667
  330. Comparison of the refolding of hen lysozyme from dimethyl-sulfoxide and guanidinium chloride. Kotik, M , Radford, SE and Dobson, CM (1995) Biochemistry, 34, 1714-1724. doi:10.1021/bi00005a028
  331. Conformation of GroEL-bound alpha-lactalbumin probed by mass-spectrometry. Robinson, CV , Gross, M , Eyles, SJ , Ewbank, JJ , Mayhew, M , Hartl, FU , Dobson, CM and Radford, SE (1994) Nature, 372, 646-651. doi:10.1038/372646a0
  332. Crystal-structure of the mutant D52S hen egg-white lysozyme with an oligosaccharide product. Hadfield, AT , Harvey, DJ , Archer, DB , MacKenzie, DA , Jeenes, DJ , Radford, SE , Lowe, G , Dobson, CM and Johnson, LN (1994) Journal of Molecular Biology, 243, 856-872. doi:10.1006/jmbi.1994.1688
  333. Kinetic consequences of the removal of a disulfide bridge on the folding of hen lysozyme. Eyles, SJ , Radford, SE , Robinson, CV and Dobson, CM (1994) Biochemistry, 33, 13038-13048. doi:10.1021/bi00248a013
  334. Far-uv circular-dichroism reveals a conformational switch in a peptide fragment from the beta-sheet of hen lysozyme. Yang, JJ , Pitkeathly, M and Radford, SE (1994) Biochemistry, 33, 7345-7353. doi:10.1021/bi00189a040
  335. Refolding of human lysozyme - a comparison with the structurally homologous hen lysozyme. Hooke, SD , Radford, SE and Dobson, CM (1994) Biochemistry, 33, 5867-5876. doi:10.1021/bi00185a026
  336. Tertiary interactions in the folding pathway of hen lysozyme - kinetic-studies using fluorescent-probes. Itzhaki, LS , Evans, PA , Dobson, CM and Radford, SE (1994) Biochemistry, 33, 5212-5220. doi:10.1021/bi00183a026
  337. Amide hydrogen-exchange in a highly denatured state - hen egg-white lysozyme in urea. Buck, M , Radford, SE and Dobson, CM (1994) Journal of Molecular Biology, 237, 247-254. doi:10.1006/jmbi.1994.1228
  338. Probing the structure of folding intermediates. Evans, PA and Radford, SE (1994) Current Opinion in Structural Biology, 4, 100-106. doi:10.1016/S0959-440X(94)90066-3
  339. Beta-lactamases - probing the mechanism of action by electrospray-ionization mass-spectrometry (ESI MS). Aplin, RT , Leung, YC , Radford, SE , Robinson, CV and WALEY, SG (1994) , , 39-47. doi:
  340. Understanding how proteins fold - the lysozyme story so far. Dobson, CM , Evans, PA and Radford, SE (1994) Trends in Biochemical Sciences, 19, 31-37. doi:10.1016/0968-0004(94)90171-6
  341. Detection of transient protein-folding populations by mass-spectrometry. Miranker, A , Robinson, CV , Radford, SE , Aplin, RT and Dobson, CM (1993) Science, 262, 896-900. doi:10.1126/science.8235611
  342. A study of D52S hen lysozyme-GlcNAc oligosaccharide complexes by NMR-spectroscopy and electrospray mass-spectrometry (vol 296, pg 153, 1992). Lumb, KJ , Aplin, RT , Radford, SE , Archer, DB , Jeenes, DJ , Lambert, N , MacKenzie, DA , Dobson, CM and Lowe, G (1993) Febs Letters, 319, 292. doi:
  343. A partially folded state of hen egg-white lysozyme in trifluoroethanol - structural characterization and implications for protein folding. Buck, M , Radford, SE and Dobson, CM (1993) Biochemistry, 32, 669-678. doi:10.1021/bi00053a036
  344. Production of n-15-labeled hen egg-white lysozyme using Aspergillus niger. ROBERTS, IN , MacKenzie, DA , Jeenes, DJ , Archer, DB , Radford, SE , Robinson, CV , Aplin, RT and Dobson, CM (1992) Biotechnology Letters, 14, 897-902. doi:10.1007/BF01020625
  345. Hydrogen-exchange in native and denatured states of hen egg-white lysozyme. Radford, SE , Buck, M , Topping, KD , Dobson, CM and Evans, PA (1992) Proteins-structure Function and Bioinformatics, 14, 237-248. doi:10.1002/prot.340140210
  346. The folding of hen lysozyme involves partially structured intermediates and multiple pathways. Radford, SE , Dobson, CM and Evans, PA (1992) Nature, 358, 302-307. doi:10.1038/358302a0
  347. Thermodynamic consequences of the removal of a disulfide bridge from hen lysozyme. Cooper, A , Eyles, SJ , Radford, SE and Dobson, CM (1992) Journal of Molecular Biology, 225, 939-943. doi:10.1016/0022-2836(92)90094-Z
  348. A study of D52S hen lysozyme-GlcNAc oligosaccharide complexes by NMR-spectroscopy and electrospray mass-spectrometry. Lumb, KJ , Aplin, RT , Radford, SE , Archer, DB , Jeenes, DJ , Lambert, N , MacKenzie, DA , Dobson, CM and Lowe, G (1992) Febs Letters, 296, 153-157. doi:10.1016/0014-5793(92)80368-Q
  349. Demonstration by NMR of folding domains in lysozyme. Miranker, A , Radford, SE , Karplus, M and Dobson, CM (1991) Nature, 349, 633-636. doi:10.1038/349633a0
  350. A photochemically induced dynamic nuclear-polarization study of denatured states of lysozyme. Broadhurst, RW , Dobson, CM , Hore, PJ , Radford, SE and Rees, ML (1991) Biochemistry, 30, 405-412. doi:10.1021/bi00216a015
  351. A 3-disulfide derivative of hen lysozyme - structure, dynamics and stability. Radford, SE , Woolfson, DN , Martin, SR , Lowe, G and Dobson, CM (1991) Biochemical Journal, 273, 211-217. doi:10.1042/bj2730211
  352. Hen egg-white lysozyme expressed in, and secreted from, Aspergillus niger is correctly processed and folded. Archer, DB , Jeenes, DJ , MacKenzie, DA , Brightwell, G , Lambert, N , Lowe, G , Radford, SE and Dobson, CM (1990) Bio-technology, 8, 741-745. doi:10.1038/nbt0890-741
  353. Antibodies against an inter-domain segment of polypeptide-chain inhibit active-site coupling in the pyruvate-dehydrogenase multienzyme complex. Radford, SE , Perham, RN , Ullrich, SJ and Appella, E (1989) Febs Letters, 250, 336-340. doi:10.1016/0014-5793(89)80750-1
  354. Conformational flexibility and folding of synthetic peptides representing an interdomain segment of polypeptide-chain in the pyruvate-dehydrogenase multienzyme complex of Escherichia coli. Radford, SE , Laue, ED , Perham, RN , Martin, SR and Appella, E (1989) Journal of Biological Chemistry, 264, 767-775. doi:
  355. Investigation of the mechanism of active-site coupling in the pyruvate-dehydrogenase multienzyme complex of Escherichia coli by protein engineering. Miles, JS , Guest, JR , Radford, SE and Perham, RN (1988) Journal of Molecular Biology, 202, 97-106. doi:10.1016/0022-2836(88)90522-0
  356. Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate-dehydrogenase multienzyme complex of Escherichia coli. Texter, FL , Radford, SE , Laue, ED , Perham, RN , Miles, JS and Guest, JR (1988) Biochemistry, 27, 289-296. doi:10.1021/bi00401a044
  357. Segmental structure and protein domains in the pyruvate-dehydrogenase multienzyme complex of Escherichia coli - genetic reconstruction invitro and 1H NMR spectroscopy. Radford, SE , Laue, ED , Perham, RN , Miles, JS and Guest, JR (1987) Biochemical Journal, 247, 641-649. doi:10.1042/bj2470641
  358. A mutant pyruvate-dehydrogenase complex of Escherichia coli deleted in the (alanine+proline)-rich region of the acetyltransferase component. Miles, JS , Guest, JR , Radford, SE and Perham, RN (1987) Biochimica Et Biophysica Acta, 913, 117-121. doi:10.1016/0167-4838(87)90319-0
  359. 2-oxo acid dehydrogenase multi-enzyme complexes - in the beginning and halfway there. Perham, RN , Packman, LC and Radford, SE (1987) Biochemical Society Symposia, , 67-81. doi:
  360. Nmr-spectroscopy studies of the flexible linkages between lipoyl domains in the pyruvate-dehydrogenase multienzyme complex from Escherichia coli. Radford, SE , Laue, ED and Perham, RN (1986) Biochemical Society Transactions, 14, 1231-1232. doi:10.1042/bst0141231
  361. The pyruvate-dehydrogenase multienzyme complex of Escherichia coli - genetic reconstruction and functional-analysis of the lipoyl domains. Graham, LD , Guest, JR , Lewis, HM , Miles, JS , Packman, LC , Perham, RN and Radford, SE (1986) Philosophical Transactions of the Royal Society A-mathematical Physical and Engineering Sciences, 317, 391-404. doi:10.1098/rsta.1986.0049
“ Proteins hold the key to the whole subject of the molecular basis of biological reactions. ”